Nontoxic Carbon Quantum Dots/g-C3N4 for Efficient Photocatalytic Inactivation of Staphylococcus aureus under Visible Light
Nontoxic Carbon Quantum Dots/g-C3N4 for Efficient Photocatalytic Inactivation of Staphylococcus aureus under Visible Light
复制标题
无毒碳量子点/g–C 3 N 4 可在可见光下有效光催化灭活金黄色葡萄球菌
DOI：
10.1002/adhm.201801534
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发表时间：
2019-05-23
影响因子：
10
通讯作者：
Xu, Feng
中科院分区：
文献类型：
--
作者：
Tang, Chenyi;Liu, Chao;Xu, Feng
抗生素的广泛使用导致抗生素耐药菌株和抗生素耐药基因在过去几十年中迅速出现。光催化灭活是一种很有前途的杀灭病原体的方法，它有效地避免了抗微生物药物引起的问题。然而，传统的光催化剂通常存在一些缺点，如原材料成本高、紫外线激发和有毒金属的潜在浸出。本研究通过在石墨氮化碳（g-C3N4）上结合碳量子点（CQDs）合成了一种无金属异质结光催化剂CQDs/g-C3N4，与纯g-C3N4相比，CQDs/g-C3N4在体外显著增强了金黄色葡萄球菌（S. aureus）的光催化失活能力。CQDs/g-C3N4在可见光下引起细胞内活性氧水平的迅速增加和细胞膜的破坏，最终导致细菌死亡。通过金黄色葡萄球菌小鼠皮肤感染模型进一步检测CQDs/g-C3N4的作用。与g-C3N4处理组相比，CQDs/g-C3N4显著降低了小鼠的细菌负荷，并促进了病变的恢复。体内和体外毒性分析表明，CQDs/g-C3N4的副作用可以忽略不计。考虑到CQDs/g-C3N4的高效光催化失活和无毒性，该可见光驱动光催化剂为金黄色葡萄球菌感染的治疗开辟了全新的途径。
The widespread use of antibiotics has caused the rapid emergence of antibiotic-resistant bacterial strains and antibiotic resistance genes in the past few decades. Photocatalytic inactivation, a promising approach for the killing of pathogens, efficiently avoids the problems induced by antimicrobial drugs. However, traditional photocatalysts usually have some disadvantages, such as high costs of raw materials, ultraviolet ray excitation, and potential leaching of toxic metals. Here, a metal-free heterojunction photocatalyst, denoted as CQDs/g-C3N4, is synthesized through incorporating carbon quantum dots (CQDs) on graphitic carbon nitride (g-C3N4), which significantly enhances photocatalytic inactivation of Staphylococcus aureus (S. aureus) compared with pure g-C3N4 in vitro. CQDs/g-C3N4 causes a rapid increase of intracellular reactive oxygen species levels and destruction of cell membranes under visible light, eventually leading to death of bacteria. The efficacy of CQDs/g-C3N4 is further examined by a mouse cutaneous infection model of S. aureus. CQDs/g-C3N4 markedly reduces the bacterial loads and prompts lesion recovery in mice, as compared with g-C3N4-treated group. In vivo and in vitro toxicity analyses show that the side effects of CQDs/g-C3N4 are negligible. Considering the efficient photocatalytic inactivation and nontoxicity of CQDs/g-C3N4, this visible-light-driven photocatalyst paves a brand new avenue for the treatment of S. aureus infection.