Kinetics of Single DNA Compaction by Hexaammine Cobalt Chloride
Kinetics of Single DNA Compaction by Hexaammine Cobalt Chloride
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六氨合氯化钴压缩单 DNA 的动力学
DOI：
10.1166/jctn.2010.1348
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发表时间：
2010
影响因子：
--
通讯作者：
李明
中科院分区：
文献类型：
--
作者：
李明
最近的单分子实验中观察到了多价阳离子压缩单个 DNA 的逐步折叠和展开动力学。然而，阶跃信号的原因尚未得到仔细研究。使用随机动力学模拟算法，我们基于最近提出的线轴模型预测了单个 DNA 延伸在各种张力下的变化的时间过程。与使用磁镊子在相同力下进行的单分子实验相比，我们发现线轴模型与折叠动力学相当一致，但与展开动力学不一致。如果从展开时间过程中删除一些罕见的、长时间等待的事件，则可以纠正展开动力学的不一致。讨论了实验的意义。
Stepwise folding and unfolding dynamics of compaction of single DNAs by multivalent cations has been observed in recent single-molecule experiments. The causes of the stepwise signals, however, have not been carefully investigated Using stochastic dynamical simulation algorism, we predict the time courses of changes in the extension of a single DNA subject to various tensions based on a spool model proposed recently. Compared with single-molecule experiments performed using a magnetic tweezer at the same forces, we find that the spool model is in reasonable agreement with the folding dynamics, but not in agreement with the unfolding dynamics The disagreement of the unfolding dynamics can be remedied if some rare, long waiting time events are removed from the unfolding time courses. The implications of the experiments are discussed.