Multiple layers of B cell memory with different effector functions
Multiple layers of B cell memory with different effector functions
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DOI：
10.1038/ni.1814
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发表时间：
2009-12-01
影响因子：
30.5
通讯作者：
Weill, Jean-Claude
中科院分区：
文献类型：
--
作者：
Dogan, Ismail;Bertocci, Barbara;Weill, Jean-Claude
记忆B细胞在其存活所需抗原的存在以及二次免疫激发后在生发中心的再次参与等方面一直存在争议。我们利用一种依赖于胞苷脱氨酶AID的新型记忆B细胞标记小鼠模型发现，用颗粒性抗原免疫后，B细胞记忆出现在几个亚群中，包括在生发中心样结构中的免疫球蛋白M阳性（IgM⁺）和IgG1⁺ B细胞簇，这些结构在免疫后可持续存在长达8个月，以及在B细胞滤泡外具有记忆表型的IgM⁺和IgG1⁺ B细胞。再次免疫激发后，IgG亚群分化为浆细胞，而IgM亚群则重新启动生发中心反应。在这个模型中，B细胞记忆以具有不同功能的多层形式出现，调和了先前相互矛盾的观点。
Memory B cells are at the center of longstanding controversies regarding the presence of antigen for their survival and their re-engagement in germinal centers after secondary challenge. Using a new mouse model of memory B cell labeling dependent on the cytidine deaminase AID, we show that after immunization with a particulate antigen, B cell memory appeared in several subsets, comprising clusters of immunoglobulin M-positive (IgM(+)) and IgG1(+) B cells in germinal center-like structures that persisted up to 8 months after immunization, as well as IgM(+) and IgG1(+) B cells with a memory phenotype outside of B cell follicles. After challenge, the IgG subset differentiated into plasmocytes, whereas the IgM subset reinitiated a germinal center reaction. This model, in which B cell memory appears in several layers with different functions, reconciles previous conflicting propositions.