Comparison of cell cycle regulatory gene mRNA in three different types of human trophoblasts and effect of transforming growth factor.
Comparison of cell cycle regulatory gene mRNA in three different types of human trophoblasts and effect of transforming growth factor.
复制标题
三种不同类型人滋养层细胞周期调控基因mRNA的比较及转化生长因子的作用。
DOI：
10.1111/j.1447-0756.2008.00753.x
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
DeLoia,JulieA
中科院分区：
文献类型：
--
作者：
Richard,CraigAH;Jones,JaquelineM;DeLoia,JulieA
目的：确定负责减少滋养层组织的增殖，合胞化和侵袭性的因素，如先兆子痫，宫内生长受限和自发性流产，是生殖生物学目前面临的挑战。这些因子，例如转化生长因子（TGF）-β，可以通过改变滋养层分化或增殖来发挥作用。因此，我们在三种常用的滋养层组织细胞模型中研究并比较了滋养层增殖和分化的特异性标志物，并研究了TGF-β对这些标志物的影响。方法：在本研究中，我们从孕早期和足月胎盘中分离了人滋养层细胞，并额外使用了人绒毛膜癌细胞（JEG-3）。研究了每种细胞类型的人绒毛膜促性腺激素（hCG）分泌的基线值和细胞周期调节因子（细胞周期蛋白E、p21、p27和p57）的相对mRNA水平。我们还研究了TGF-β对这些参数的影响。结果：hCG的定量和纵向生产在三种细胞类型之间存在差异。在整个培养期间，在每种细胞类型内以及所有细胞类型之间，细胞周期蛋白E、p21、p27和p57 mRNA的量显著不同。每种滋养层类型都表现出响应于TGF-β的hCG分泌减少。TGF-β对任何细胞类型的细胞周期mRNA没有表现出一致的影响。结论：我们能够表征和比较hCG的差异产生，以及早期滋养层细胞、足月滋养层细胞和绒毛膜癌细胞的细胞周期相关mRNA的差异表达。hCG的产生被TGF-β改变，尽管mRNA水平没有被TGF-β显著改变。
Aim:Identifying the factors responsible for reducing the proliferation, syncytialization, and invasiveness of trophoblast tissues, as seen with preeclampsia, intrauterine growth restriction, and spontaneous miscarriage, is a current challenge in reproductive biology. These factors, transforming growth factor (TGF)‐β as an example, can work by altering trophoblast differentiation or proliferation. We therefore investigated and compared specific markers of trophoblast proliferation and differentiation in three commonly used trophoblast tissue cell models, and also investigated the influence of TGF‐β on these markers.Methods:In this study, we isolated human trophoblasts from first trimester and term placentas, and additionally used human choriocarcinoma cells (JEG‐3). Baseline values of human chorionic gonadotropin (hCG) secretion and relative mRNA levels of cell cycle regulators (cyclin E, p21, p27, and p57) were investigated for each cell type. We also investigated the influence of TGF‐β on these parameters.Results:Quantitative and longitudinal production of hCG differed between the three cell types. Significantly different amounts of cyclin E, p21, p27, and p57 mRNA were demonstrated within each cell type, as well as between all the cell types, throughout the culture time period. Each trophoblast type demonstrated a reduction of hCG secretion in response to TGF‐β. TGF‐β did not show a consistent effect on the cell cycle mRNA of any of the cell types.Conclusion:We were able to characterize and compare the differential production of hCG, as well as the differential expression of cell cycle‐associated mRNA of early trophoblasts, term trophoblasts, and choriocarcinoma cells. The production of hCG was altered by TGF‐β, although mRNA levels were not markedly altered by TGF‐β.