Red blood cells aggregability measurement of coagulating blood in extracorporeal circulation system with multiple-frequency electrical impedance spectroscopy
Red blood cells aggregability measurement of coagulating blood in extracorporeal circulation system with multiple-frequency electrical impedance spectroscopy
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DOI：
10.1016/j.bios.2018.04.020
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发表时间：
2018-07-30
影响因子：
12.6
通讯作者：
Takei, Masahiro
中科院分区：
文献类型：
--
作者：
Li, Jianping;Sapkota, Achyut;Takei, Masahiro
本文研究了脉动流条件下体外循环中红细胞聚集性A（G）的变化。利用来自多频电阻抗谱的弛豫频率f（c）来获得RBC聚集性AG。与其他方法相比，多频电阻抗法更容易实现对生物组织的无创测量，且速度快、穿透性好。实验结果表明，红细胞聚集性AG在无凝血福尔斯中随血栓形成而下降，而在无凝血中几乎保持不变，与活化凝血时间（ACT）、纤维蛋白原浓度（4，）试验结果一致。提出了修正的Hanai公式，定量分析了红细胞聚集对多频电阻抗测量的影响。红细胞聚集性AG的降低与血液凝固反应有关，这表明高速、紧凑、廉价的在线血栓测量生物传感器在体外循环系统中的可行性。
Red blood cells (RBCs) aggregability A(G) of coagulating blood in extracorporeal circulation system has been investigated under the condition of pulsatile flow. Relaxation frequency f(c) from the multiple-frequency electrical impedance spectroscopy is utilized to obtain RBCs aggregability AG. Compared with other methods, the proposed multiple-frequency electrical impedance method is much easier to obtain non-invasive measurement with high speed and good penetrability performance in biology tissues. Experimental results show that, RBCs aggregability AG in coagulating blood falls down with the thrombus formation while that in non-coagulation blood almost keeps the same value, which has a great agreement with the activated clotting time (ACT) fibrinogen concertation (4,) tests. Modified Hanai formula is proposed to quantitatively analyze the influence of RBCs aggregation on multiple-frequency electrical impedance measurement. The reduction of RBCs aggregability AG is associated with blood coagulation reaction, which indicates the feasibility of the high speed, compact and cheap on-line thrombus measurement biosensors in extracorporeal circulation systems.