Seroepidemiology of the human polyomaviruses
Seroepidemiology of the human polyomaviruses
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DOI：
10.1099/vir.0.18842-0
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发表时间：
2003-06-01
影响因子：
3.8
通讯作者：
Dillner, J
中科院分区：
文献类型：
--
作者：
Stolt, A;Sasnauskas, K;Dillner, J
为了评估连续样品中多瘤病毒抗体随时间变化的稳定性以及多瘤病毒感染的发病率和年龄特异性患病率，我们使用纯化的酵母表达的病毒样颗粒（VLP）建立了酶免疫测定法（EIA），该病毒样颗粒（VLP）含有JC病毒（JCV）的VP1主要衣壳蛋白以及BK病毒（BKV）的AS和SB毒株。选择了 150 名芬兰妇女的随机子样本，这些妇女在怀孕的前三个月采集了血清样本，并在 5 年的随访期内进行了第二次怀孕，并按第一次怀孕的年龄分组。第一次和第二次怀孕期间采集的样本中的多瘤病毒抗体水平相似（BKV SB 的相关系数为 0(.)93，JCV 的相关系数为 0-94）。对 290 名 1-13 岁瑞典儿童的血清样本进行分析，按年龄分组，间隔 2 年，结果表明，BKV 血清阳性率随着儿童年龄的增加而迅速增加，在 7-9 岁时血清阳性率达到 98%，随后略有下降。 JCV 血清阳性率随着年龄的增长而缓慢增加，在 25 岁以上的母亲中，阳性率达到 72%。使用这种基于 VLP 的 EIA 测量的人类多瘤病毒的年龄特异性血清流行率与之前通过其他方法进行的血清调查相似。抗体随时间的稳定性表明多瘤病毒血清阳性是累积病毒暴露的有效标志，因此基于多瘤 VLP 的 EIA 可能有助于这些病毒的流行病学研究。
To assess the stability of polyomavirus antibodies in serial samples over time and the incidence and age-specific prevalence of polyomavirus infections, we established enzyme immunoassays (EIAs) using purified yeast-expressed virus-like particles (VLPs) containing the VP1 major capsid proteins of JC virus (JCV) and the AS and SB strains of BK virus (BKV). A random subsample of 150 Finnish women who had serum samples taken during the first trimester of pregnancy and had a second pregnancy during a 5 year follow-up period was selected, grouped by age of first pregnancy. The polyomavirus antibody levels were similar in samples taken during the first and second pregnancies (correlation coefficient 0(.)93 for BKV SB and 0-94 for JCV). Analysis of serum samples from 290 Swedish children aged 1-13 years, grouped by age in 2 year intervals, demonstrated that BKV seropositivity increased rapidly with increasing age of the children, reaching 98 % seroprevalence at 7-9 years of age, followed by a minor decrease. JCV seroprevalence increased only slowly with increasing age and reaching 72 % positivity among mothers > 25 years of age. The age-specific seroprevalence of the human polyomaviruses measured using this VLP-based EIA was similar to previous serosurveys by other methods. The stability of the antibodies over time indicates that polyomavirus seropositivity is a valid marker of cumulative virus exposure, and polyoma VLP-based EIAs may therefore be useful for epidemiological studies of these viruses.