Cell-cycle analysis of insulin binding and internalization on mouse melanoma cells.
Cell-cycle analysis of insulin binding and internalization on mouse melanoma cells.
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DOI：
10.1083/jcb.88.1.241
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发表时间：
1981-01
期刊：
影响因子：
--
通讯作者：
Fuller BB
中科院分区：
文献类型：
--
作者：
Shimizu N;Shimizu Y;Fuller BB
在细胞周期的不同阶段（M、G1、S和G2）研究了125 I标记的胰岛素与Cloudman S- 91小鼠黑素瘤细胞（CCL 53.1）表面受体的结合。胰岛素结合活性持续存在于细胞周期中，但最高的活性在S期。一旦在细胞周期的任何阶段与细胞表面受体结合，胰岛素就会被内化和降解，推测是通过溶酶体途径。黑色素细胞刺激素不影响黑色素细胞的胰岛素指数活性。
Binding of 125I-labeled insulin to the surface receptors of Cloudman S- 91 mouse melanoma cells (CCL 53.1) was studied at various phases (M, G1, S, and G2) in the cell cycle. Insulin-binding activity was persistently present during the cell cycle but the highest activity was noted at the S-phase. The insulin once bound to the cell surface receptors at any phase of the cell cycle was internalized and degraded, presumably through a lysosomal pathway. Insulin-indexing activity of melanoma cells was not affected by melanocyte-stimulating hormone.