Bone morphogenesis and surface charge.
Bone morphogenesis and surface charge.
复制标题
骨形态发生和表面电荷。
DOI：
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发表时间：
1976
影响因子：
4.2
通讯作者：
C. Eriksson
中科院分区：
文献类型：
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作者：
C. Eriksson
已在生理溶液中测量了经过不同处理的具有或不具有骨形态发生活性的骨样本的电泳迁移率。骨形态发生原理似乎与植入物上的最小负表面电荷有关，该表面电荷对应于盐水中正常未脱矿质皮质骨的表面电荷。 2 度 0.6 N HCl 中皮质骨的脱矿质持续增加骨负表面电荷，并从生物学角度产生具有形态发生活性的植入物。这表明，通过负植入物周围带正电荷的离子层的介导，带负电荷的间充质细胞能够与植入物的表面非常紧密地接触。预计结合力与植入物的负电程度成正比。然而，具有正 zeta 电位的植入物将被带有净负电荷的离子包围，因此不利于间充质细胞与植入物紧密接触的稳定定位。与未经处理的皮质骨的表面负电荷相对应的负表面电荷显然处于允许这种紧密接触足够长的时间以进行分化的临界点。
The electrophoretic mobility of variously treated bone specimens, with and without bone morphogenetic activity, has been measured in physiological solutions. The Bone Morphogenetic Principle appears to be associated with a minimum negative surface charge on the implant that corresponds to that of normal undemineralized cortical bone in saline. Demineralization of cortical bone in 0.6 N HCI at 2 degrees consistently increases the bone negative surface charge, and biologically produces an implant with morphogenetic activity. It is suggested that through the mediation of the positively charges ionic layer surrounding the negative implant that the negatively charged mesenchymal cell is able to reside in very close contact with the surfaces of the implant. The binding force can be expected to be directly proportional to the degree of negativity of the implant. However, implants with a positive zeta potentaial will be surrounded by ions with a net negative charge so discouraging the stable positioning of mesenchymal cells in close contact with the implant. The negative surface charge corresponding to that of untreated cortical bone is apparently borderline in permitting such a close contact for a sufficiently long period of time for differentiation to follow.