Functional heterogeneity of the Lyb-5- B cell subpopulation: mutant xid B cells and normal Lyb-5- B cells differ in their responsiveness to phenol-extracted lipopolysaccharide.
Functional heterogeneity of the Lyb-5- B cell subpopulation: mutant xid B cells and normal Lyb-5- B cells differ in their responsiveness to phenol-extracted lipopolysaccharide.
复制标题
Lyb-5-B细胞亚群的功能异质性：突变xid B细胞和正常Lyb-5-B细胞对苯酚提取的脂多糖的反应性不同。
作者：
S. Ono;L. Yaffe;J. Ryan;A. Singer
在本研究中，苯酚提取的脂多糖（LPS（苯酚））和丁醇提取的LPS（LPS（丁醇））刺激的反应被用来评估xid B细胞可能与正常小鼠中存在的LyB-5- B细胞不同的可能性。发现xid B细胞仅对LPS（丁醇）反应良好，而正常B细胞对LPS（丁醇）和LPS（苯酚）反应良好。因此，LPS（丁醇）似乎是TI-1抗原，LPS（苯酚）似乎是TI-2抗原。然而，与经典的TI-2反应相反，LPS（苯酚）刺激的反应没有表现出对辅助细胞的严格要求。此外，如果LPS（苯酚）是经典的TI-2抗原，则它应该仅激活LyB-5+ B细胞。为了确定正常B细胞对LPS（苯酚）的反应性是否至少部分是由于正常LyB-5- B细胞的刺激，评估了正常新生儿B细胞和已用抗LyB-5.1 + C预处理的正常成人B细胞的反应性。发现正常新生儿B细胞和正常成人LyB-5- B细胞均对LPS（苯酚）反应良好。因此，即使LPS（苯酚）不刺激xid B细胞，这些数据证明LPS（苯酚）不同于其它TI-2抗原。更重要的是，这些数据还表明，xid B细胞和正常的LyB-5- B细胞是不相同的。假设正常的LyB-5- B细胞亚群是异质的，由LyB-5（1）-和LyB-5（2）-B细胞亚群组成，其中xid突变阻断LyB-5（1）-B细胞分化为LyB-5（2）-B细胞。
In the present study, responses stimulated by phenol-extracted lipopolysaccharide (LPS(phenol)) and butanol-extracted LPS (LPS(butanol)) were used to assess the possibility that xid B cells might not be identical to the Lyb-5- B cells present in normal mice. It was found that xid B cells responded well only to LPS(butanol) whereas normal B cells responded well to both LPS(butanol) and LPS(phenol). Thus, LPS(butanol) appeared to be a TI-1 antigen and LPS(phenol) appeared to be a TI-2 antigen. In contrast to classical TI-2 responses, however, responses stimulated by LPS(phenol) did not exhibit a stringent requirement for accessory cells. Furthermore, if LPS(phenol) were a classical TI-2 antigen, it should only activate Lyb-5+ B cells. To determine if the responsiveness of normal B cells to LPS(phenol) were due, at least in part, to the stimulation of normal Lyb-5- B cells, the responsiveness of normal neonatal B cells and normal adult B cells that had been pretreated with anti-Lyb-5.1 + C was assessed. It was found that both normal neonatal B cells and normal adult Lyb-5- B cells did respond well to LPS(phenol). Thus, even though LPS(phenol) does not stimulate xid B cells, these data demonstrate that LPS(phenol) is different from other TI-2 antigens. More importantly, these data also demonstrate that xid B cells and normal Lyb-5- B cells are not identical. It is hypothesized that the normal Lyb-5- B cell subpopulation is heterogeneous, consisting of an Lyb-5(1)- and an Lyb-5(2)-B cell subset with the xid mutation blocking the differentiation of Lyb-5(1)-B cells into Lyb-5(2)-B cells.