Chemokines are elevated in plasma after strenuous exercise in humans
Chemokines are elevated in plasma after strenuous exercise in humans
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DOI：
10.1007/s004210170012
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发表时间：
2001-03-01
影响因子：
3
通讯作者：
Pedersen, BK
中科院分区：
文献类型：
--
作者：
Ostrowski, K;Rohde, T;Pedersen, BK
在过去的几年里，趋化因子得到了很大的关注。趋化因子受体是使靶标允许人类免疫缺陷病毒（HIV）感染所必需的，高浓度的趋化因子已被证明可以防止HIV疾病向死亡的进展。在本研究中，我们研究了剧烈运动诱导白细胞介素(IL)-8、巨噬细胞炎症蛋白(MIP)-1 α和MLP-1 β趋化因子血浆浓度升高的能力。8名男性运动员完成了1997年哥本哈根马拉松比赛。在跑步前、跑步后和4小时恢复期每30分钟采集一次血样。采用酶联免疫吸附法测定血浆趋化因子浓度。IL-8、MIP-1 α和mtp - β浓度在运行后0.5 h达到峰值，分别增加6.7倍、3.5倍和4.1倍。运动后血浆中趋化因子浓度升高可能对hev感染者有影响；这种可能性需要进一步调查。通过与细胞表面蛋白CD4和称为辅助受体的其他分子相互作用感染靶细胞，这些分子已被确定为趋化因子受体家族的成员（Feng et al. 1996）。CD4和趋化因子受体的同时表达是使靶鳗易受HIV感染的必要条件。趋化因子可以通过竞争性抑制来阻止艾滋病毒与趋化因子受体的结合，因此有人认为，影响趋化因子血浆浓度的因素可能有可能影响艾滋病毒疾病的进展（Zagury等人，1998年）。我们和其他人之前已经表明，运动可以诱导炎症相关细胞因子的产生，尤其是IL-6和IL-1ra （Drenth et al. 1995; Nieman 1997; Ostrowski et al. 1998a, b, 1999）。本研究探讨剧烈运动在多大程度上影响趋化因子白介素(IL)-8、巨噬细胞炎症蛋白(MIP)-1 α和MTP-1 β的血浆浓度。
During the last few years much attention has been paid to the chemokines. Chemokine receptors are necessary to render a target permissive for infection by the human immunodeficiency virus (HIV) and high concentrations of chemokines have been shown to protect against the progression of HIV disease towards death. In the present study, we investigated the capability of strenuous exercise to induce elevated plasma concentrations of the chemokines interleukin (IL)-8, macrophage inflammatory protein (MIP)-1 alpha and MLP-1 beta Eight male athletes completed the Copenhagen Marathon 1997. Blood was sampled before, immediately after the run and every 30 min during a 4 h recovery period. Plasma chemokine concentrations were measured using enzyme-linked immunosorbent assays. The IL-8, MIP-1 alpha and MTP-beta concentrations all peaked 0.5 h after the run when they were 6.7-fold, 3.5-fold and 4.1-fold increased, respectively. The elevated concentrations of chemokines in plasma after exercise could have implications for HEV-infected individuals; a possibility that needs further investigation. infects target cells by interacting with the cell surface protein CD4 and additional molecules, termed coreceptors, which have been identified as members of the chemokine receptor family (Feng et al. 1996). Con-comitant expression of CD4 and a chemokine receptor is necessary to render a target eel susceptible to infection with HIV. Chemokines can prevent binding of HIV to the chemokine receptor by competitive inhibition and it has been suggested that factors influencing the plasma concentrations of chemokines may therefore have the potential to affect the progression of HIV disease (Zagury et al. 1998). We and others have previously shown that exercise induces production of the cytokines involved in inflammation, especially IL-6 and IL-1ra (Drenth et al. 1995; Nieman 1997; Ostrowski et al. 1998a, b, 1999). The present study investigated to what extent strenuous exercise affects the plasma concentrations of the chemokines interleukin (IL)-8, macrophage inflammatory protein (MIP)-1 alpha and MTP-1 beta.