ROLE OF MAST-CELLS IN ANAPHYLAXIS - EVIDENCE FOR THE IMPORTANCE OF MAST-CELLS IN THE CARDIOPULMONARY ALTERATIONS AND DEATH INDUCED BY ANTI-IGE IN MICE
ROLE OF MAST-CELLS IN ANAPHYLAXIS - EVIDENCE FOR THE IMPORTANCE OF MAST-CELLS IN THE CARDIOPULMONARY ALTERATIONS AND DEATH INDUCED BY ANTI-IGE IN MICE
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DOI：
10.1172/jci114025
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发表时间：
1989-04-01
影响因子：
15.9
通讯作者：
DRAZEN, JM
中科院分区：
文献类型：
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作者：
MARTIN, TR;GALLI, SJ;DRAZEN, JM
本研究采用肥大细胞缺陷型WBB 6 F1-W/Wv和WCB 6 F1-S1/S1 d小鼠及同系正常（+/+）小鼠，观察静脉注射山羊抗鼠IgE对小鼠心率（HR）、肺动态顺应性（Cdyn）、肺传导性（GL）及存活率的影响。在WBB 6 F1-+/+和WCB 6 F1-+/+小鼠中，抗IgE诱导气管支气管肥大细胞广泛脱颗粒，以及HR显著升高、Cdyn和GL显著降低，在某些情况下导致死亡。相比之下，W/Wv和S1/S1 d小鼠表现出很少或没有病理生理反应，并且在用抗IgE激发后没有死亡。在W/Wv小鼠中，通过静脉内施用来自同源+/+小鼠（+/+ BM → BM）的骨髓细胞，用肥大细胞重建。W/Wv小鼠），抗IgE诱导广泛的肥大细胞脱颗粒，以及与WBB 6 F1-+/+小鼠中观察到的相似的病理生理学反应和死亡率。这些发现表明肥大细胞在抗IgE诱导的过敏反应相关的心肺变化和死亡率的发展中起关键作用。
We used genetically mast cell-deficient WBB6F1-W/Wv and WCB6F1-S1/S1d mice and the congenic normal (+/+) mice to investigate the effects of intravenous infusion of goat antimouse IgE on heart rate (HR), pulmonary dynamic compliance (Cdyn), pulmonary conductance (GL), and survival. In WBB6F1-+/+ and WCB6F1-+/+ mice, anti-IgE induced extensive degranulation of tracheobronchial degranulation of tracheobronchial mast cells, as well as significant elevation of HR, significant reductions in Cdyn and GL and, in some cases, death. In contrast, W/Wv and S1/S1d mice exhibited little or no pathophysiological responses and no mortality after challenge with anti-IgE. In W/Wv mice reconstituted with mast cells by intravenous administration of bone marrow cells derived from congenic +/+ mice (+/+ BM .fwdarw. W/Wv mice), anti-IgE induced extensive mast cell degranulation, as well as pathophysiological responses and mortality similar to those observed in WBB6F1-+/+ mice. These findings suggest a critical role for mast cells in the development of the cardiopulmonary changes and mortality associated with anti-IgE-induced anaphylaxis.