Proteomic analysis of peach endocarp and mesocarp during early fruit development
Proteomic analysis of peach endocarp and mesocarp during early fruit development
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DOI：
10.1111/j.1399-3054.2011.01479.x
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发表时间：
2011-08-01
影响因子：
6.4
通讯作者：
Wang, You-Nian
中科院分区：
文献类型：
--
作者：
Hu, Hao;Liu, Yong;Wang, You-Nian
本文采用蛋白质组学方法对桃果核的发育和形成进行了研究。在果实发育早期（开花后28 ~ 59天）内果皮和中果皮中鉴定出68种不同表达模式的蛋白，其中大多数参与初级或次级代谢。与内果皮中与初级代谢相关的大多数蛋白表达下调相反，丙酮酸脱氢酶（PDH）的表达出乎意料地呈指数增长。其表达量与木质素含量呈指数增长呈线性正相关（R = 0.940），表明PDH可能在果皮内木质素化过程中发挥作用。我们的数据还揭示了参与木质素和类黄酮途径的酶的不同时空表达，提供了蛋白质组水平的证据，支持这两个途径在内果皮发育过程中竞争的假设。此外，我们观察到内果皮特异性氧化应激，并提出它可能是激活内果皮木质化和随后的程序性细胞死亡的刺激因素。
The development of the stone and formation of peach (Prunus persica) fruit were explored in this work using a proteomic approach. Sixty-eight proteins with different expression patterns were identified in both the endocarp and mesocarp during early fruit development (from 28 to 59 days after flowering) and the majority were involved in primary or secondary metabolism. In contrast to most proteins associated with primary metabolism in the endocarp, whose expression is down-regulated, expression of pyruvate dehydrogenase (PDH) unexpectedly increased exponentially. Moreover, its expression pattern was linearly positively correlated with the exponentially growing lignin content (R = 0.940), which suggests that PDH may play a role in endocarp lignification. Our data also revealed different spatiotemporal expressions of enzymes involved in the lignin and flavonoid pathways that provided proteome-level evidence to support the hypothesis that these two pathways are competitive during endocarp development. In addition, we observed endocarp-specific oxidative stress and propose that it may act as a stimulating factor in activating lignification and subsequent programmed cell death in the endocarp.