Visualization of iron-rich subcortical structures in non-human primates in vivo by quantitative susceptibility mapping at 3T MRI.
Visualization of iron-rich subcortical structures in non-human primates in vivo by quantitative susceptibility mapping at 3T MRI.
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通过 3T MRI 定量磁化率图对非人类灵长类动物体内富含铁的皮层下结构进行可视化。
DOI：
10.1016/j.neuroimage.2021.118429
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发表时间：
2021
期刊：
影响因子：
5.7
通讯作者：
Hikosaka,Okihide
中科院分区：
文献类型：
--
作者：
Yoshida,Atsushi;Ye,FrankQ;Yu,DavidK;Leopold,DavidA;Hikosaka,Okihide
磁共振成像（MRI）是目前非人灵长类（NHP）神经科学领域的重要工具。结构MRI扫描使用t1加权（T1w）或t2加权（T2w）图像提供解剖学信息，特别是涉及基底节区和小脑等深层结构的实验。然而，对于某些皮质下结构，T1w和T2w图像对比度不足以检测重要的解剖细节。为了更好地观察猕猴大脑中的这些结构，我们应用了一种相对较新的方法，称为定量磁化率制图（QSM），该方法基于局部组织磁化率增强组织对比度。QSM显著改善了重要结构的可视化，包括侧侧苍白球（VP）、苍白球外段和内段（GPe和GPi）、黑质（SN）、基底节区丘脑下核（STN）和小脑齿状核（DN）。我们通过系统地比较QSM图像相对于相应的T1w和T2w图像的对比噪声比（CNRs）来量化这种对比度增强。此外，一些结构的QSM值与猕猴被试的年龄相关。这些结果表明，QSM方法是一种简单而有用的工具，可以清晰地可视化皮质下结构的细节，而传统的扫描序列是看不见的。
Magnetic resonance imaging (MRI) is now an essential tool in the field of neuroscience involving non-human primates (NHP). Structural MRI scanning using T1-weighted (T1w) or T2-weighted (T2w) images provides anatomical information, particularly for experiments involving deep structures such as the basal ganglia and cerebellum. However, for certain subcortical structures, T1w and T2w image contrasts are insufficient for their detection of important anatomical details. To better visualize such structures in the macaque brain, we applied a relatively new method called quantitative susceptibility mapping (QSM), which enhances tissue contrast based on the local tissue magnetic susceptibility. The QSM significantly improved the visualization of important structures, including the ventral pallidum (VP), globus pallidus external and internal segments (GPe and GPi), substantia nigra (SN), subthalamic nucleus (STN) in the basal ganglia and the dentate nucleus (DN) in the cerebellum. We quantified this the contrast enhancement by systematically comparing of contrast-to-noise ratios (CNRs) of QSM images relative to the corresponding T1w and T2w images. In addition, QSM values of some structures were correlated to the age of the macaque subjects. These results identify the QSM method as a straightforward and useful tool for clearly visualizing details of subcortical structures that are invisible with more traditional scanning sequences.