Structure of perosamine-containing polysaccharide, a component of the sheath of Thiothrix fructosivorans.
Structure of perosamine-containing polysaccharide, a component of the sheath of Thiothrix fructosivorans.
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含过胺多糖的结构，是食果糖硫丝菌鞘的组成部分。
DOI：
10.1016/j.ijbiomac.2013.04.013
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发表时间：
2013
影响因子：
8.2
通讯作者：
Takeda M.
中科院分区：
文献类型：
--
作者：
Kondo K;Umezu T;Shimura S;Narizuka R;Koizumi J;Mashima T;Katahira M;Takeda M.
采用异养培养方法培养了一种成鞘和硫氧化细菌——果糖硫thrix fructosivorans。鞘是一种细胞外微管，用溶菌酶、十二烷基硫酸钠和氢氧化钠选择性去除细胞制备。固体13c核磁共振（NMR）显示鞘是由具有乙酰基和甲基的聚糖组装而成的。当鞘被去乙酰化时，原有的微管结构丢失，鞘在酸性条件下变得可溶，揭示了乙酰基在维持鞘结构中的重要性。气相液相色谱法检测鞘酸水解产物中等摩尔d-葡萄糖、d-氨基葡萄糖和l-焦。除了这些糖外，还通过高效液相色谱分析检测到β-GlcN-(1→4)- glc和未知糖。利用1H和13c NMR谱鉴定了一种由4-脱氧-4-氨基鼠李糖（perosamine, Rha4N）和focus组成的双糖。由该双糖制备的n -乙酰-perosamine具有极化和d-构型。先前未知的双糖被发现是α-d-Rhap4N-(1→3)-d- fuc。1h和13c NMR分析发现，脱乙酰成鞘多糖具有[→4)-β-d- glcpn -(1→4)-β-d- glcp -(1→]n主链，在Glc的3位上附着α-d-Rhap4N-(1→3)-α-l- fucp -（1→）双糖侧链。
A sheath-forming and sulfur-oxidizing bacterium, Thiothrix fructosivorans, was heterotrophically cultured. The sheath, which is an extracellular microtube, was prepared by selectively removing the cells using lysozyme, sodium dodecyl sulfate, and sodium hydroxide. Solid-state13C-nuclear magnetic resonance (NMR) spectrum revealed that the sheath is assembled from a glycan possessing acetyl and methyl groups. When the sheath was deacetylated, the original microtube structure was lost and the sheath became soluble under acidic conditions, revealing the importance of acetyl groups in maintaining the sheath structure. Equimolar d-glucose, d-glucosamine, and l-fucose were detected in the acid hydrolysate of the sheath by gas liquid chromatography. In addition to these sugars, β-GlcN-(1→4)-Glc and unidentified sugar were detected by analyzing the hydrolysate using high-performance liquid chromatography analysis.1H and13C NMR spectroscopy was used to identify a disaccharide composed of 4-deoxy-4-aminorhamnose (perosamine, Rha4N) and fucose. N-Acetyl-perosamine prepared from the disaccharide was polarimetric and exhibited a d-configuration. The previously unidentified disaccharide was found to be α-d-Rhap4N-(1→3)-d-Fuc. According to1H and13C NMR analyses, the deacetylated sheath-forming polysaccharide was found to h have a main chain of [→4)-β-d-GlcpN-(1→4)-β-d-Glcp-(1→]n, to which disaccharide side chains of α-d-Rhap4N-(1→3)-α-l-Fucp-(1→ were attached at position 3 of Glc.