Two-stage hormonal control of type IV collagen mRNA levels during differentiation of F9 teratocarcinoma cells.
Two-stage hormonal control of type IV collagen mRNA levels during differentiation of F9 teratocarcinoma cells.
复制标题
F9 畸胎癌细胞分化过程中 IV 型胶原蛋白 mRNA 水平的两阶段激素控制。
DOI：
10.1016/0012-1606(85)90005-3
复制
发表时间：
1985
影响因子：
2.7
通讯作者：
Strickland,S
中科院分区：
文献类型：
--
作者：
Marotti,KR;Brown,GD;Strickland,S
以维甲酸和二丁基camp诱导分化的F9畸胎瘤细胞为材料，制备了小鼠IV型胶原蛋白相关cDNA克隆。该cDNA克隆已被用于研究IV型胶原mRNA在分化过程中的调控。在未处理的F9细胞中，这种mRNA的水平很低，在维甲酸处理后，这种mRNA的水平大幅增加，在添加二丁基camp后，这种mRNA的水平进一步增加。相反，二丁基camp对未暴露于视黄酸的细胞的mRNA水平没有影响。这些结果表明，这两种化合物以顺序的方式调节IV型胶原mRNA的稳态水平。该cDNA克隆将允许对类维生素a和环AMP两阶段调节胶原表达的机制进行详细检查。
A cDNA clone related to mouse Type IV collagen has been prepared from F9 teratocarcinoma cells induced to differentiate with retinoic acid and dibutyryl-cAMP. This cDNA clone has been used to investigate the regulation of Type IV collagen mRNA during differentiation. The level of this mRNA is very low in untreated F9 cells, increases substantially after treatment of the cells with retinoic acid, and is further increased by addition of dibutyryl-cAMP. In contrast, dibutyryl-cAMP has no effect on the mRNA level in cells that have not been previously exposed to retinoic acid. These results demonstrate that these two compounds regulate in a sequential manner the steady-state level of Type IV collagen mRNA. This cDNA clone should allow a detailed examination of the mechanism of the two-stage regulation of collagen expression by retinoids and cyclic AMP.