Rhizobium rhizogenes-mediated root proliferation in Cd/Zn hyperaccumulator Sedum alfredii and its effects on plant growth promotion, root exudates and metal uptake efficiency.
Rhizobium rhizogenes-mediated root proliferation in Cd/Zn hyperaccumulator Sedum alfredii and its effects on plant growth promotion, root exudates and metal uptake efficiency.
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DOI：
10.1016/j.jhazmat.2021.127442
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发表时间：
2021-10
影响因子：
13.6
通讯作者：
Z. A. Sahito;A. Zehra;Shaoning Chen;Song-lin Yu;Lin Tang;Zarina Ali;S. Hamza;M. Irfan;T. Abbas;Zhenli He;Xiaoe Yang
中科院分区：
文献类型：
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作者：
Z. A. Sahito;A. Zehra;Shaoning Chen;Song-lin Yu;Lin Tang;Zarina Ali;S. Hamza;M. Irfan;T. Abbas;Zhenli He;Xiaoe Yang
本研究建立了发根根瘤菌（Rhizobium rhizogenes）介导的佛雷景天（Sedumalfredi）根增殖体系。20株R.筛选发根植物的根增殖。不同菌株对植株形态性状的影响差异极显著（P< 0.01）。菌株AS 12556的根鲜重最高（3.236 g/株）。此外，不同发根根瘤菌介导根系分泌物中有机酸、酒石酸（TA）、琥珀酸（SA）、苹果酸（MA）、柠檬酸（CA）、草酸（OA）的化学组成、pH值、总氮（TN）、总有机碳（TOC）和可溶性糖的含量也存在显著差异（P< 0.05）。此外，用不同浓度的Cd（25、50和75 μM）和Zn（100、200和500 μM）进行了一系列水培实验，以评估用根瘤菌增殖的根的植物提取效率。几种植物增殖的根表现出增强的增长和提高金属提取效率。通过对5个菌株（LBA 9402、K599、AS 12556、MSU 440和C58 C1）的筛选，确定了5个菌株为东南景天根增殖的潜在菌株。发根菌株AS 12556通过外源植物化学物质的产生，促进东南景天根系的增殖，提高地上部生物量，降低氧化损伤，提高植物提取效率，从而改善了东南景天对Cd/Zn的吸收。因此，它已被选为S的潜在微生物伴侣。alfrediito开发广泛的生根系统，以获得更好的生长和增强植物修复潜力。结果表明，R. rhizogenesmediated根增殖系统可用于优化污染土壤中金属的提取。
In this study, Rhizobium rhizogenes-mediated root proliferation system inSedumalfrediihas been established. Twenty strains ofR. rhizogeneswere screened for root proliferation. A significant difference (P< 0.01) was observed in plant morphological characters under influence of different bacterial strains. The highest root fresh weight (3.236 g/plant) was observed with strain AS12556. Furthermore, significant difference (P< 0.05) was observed in the chemical composition of organic acids, Tartaric acid (TA), Succinic acid (SA), Malic acid (MA), Citric acid (CA) and Oxalic acid (OA), pH, Total Nitrogen (TN), Total Organic Carbon (TOC) and soluble sugars in root exudates with differentR.rhizogenesmediated roots. Furthermore, a series of hydroponics experiments were conducted with varying concentrations of Cd (25, 50 and 75 µM) and Zn (100, 200 and 500 µM) to assess the phytoextraction efficiency of proliferated roots with Rhizobium. Several plants with proliferated roots showed enhanced growth and improved metal extraction efficiency. Five strains (LBA 9402, K599, AS12556, MSU440 and C58C1) were identified as potential strains for root proliferation inSedum alfredii.R. rhizogenesstrain AS12556 improved Cd/Zn phytoextraction by exogenous production of phytochemicals to promote root proliferation, improved shoot biomass, lowered oxidative damage and enhanced phytoextraction efficiency inS. alfredii. Therefore, it has been selected as a potential microbial partner ofS. alfrediito develop extensive rooting system for better growth and enhanced phytoremediation potential. Results suggest thatR. rhizogenesmediated root proliferation system can be used for optimizing metal extraction from contaminated soils.