Human DNA (cytosine-5)methyltransferase selectively methylates duplex DNA containing mispairs.
Human DNA (cytosine-5)methyltransferase selectively methylates duplex DNA containing mispairs.
复制标题
人类 DNA (胞嘧啶-5) 甲基转移酶选择性地甲基化含有错配的双链 DNA。
DOI：
10.1093/nar/15.17.6899
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发表时间：
1987
影响因子：
14.9
通讯作者：
Baker,DJ
中科院分区：
文献类型：
--
作者：
Smith,SS;Hardy,TA;Baker,DJ
The presence of the C.C mispair in a defined duplex oligodeoxy-nucleotide enhanced its capacity to serve as a substrate for highly purified human DNA methyltransferase. Analysis of tritiated reaction products showed that the C.C mispair acted as a “methylation acceptor” in that it was itself rapidly methylated. The m5C.G base pair also enhanced the capacity of the oligodeoxynucleotide to serve as a substrate for the enzyme. However, this complementary base pair was found to act as a “methylation director”. That is, the presence of the m5C in one strand induced the enzyme to rapidly methylate at the cytosine residue on the opposite strand in an adjacent C.G base pair.
