Creating new genes by plasmid recombination in Escherichia coli and Bacillus subtilis
Creating new genes by plasmid recombination in Escherichia coli and Bacillus subtilis
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DOI：
10.1128/aem.71.11.7607-7609.2005
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发表时间：
2005-11-01
影响因子：
4.4
通讯作者：
Petit, MA
中科院分区：
文献类型：
--
作者：
Gomez, A;Galic, T;Petit, MA
基因改组是一种从不同的序列开始创造具有有趣的新特征的蛋白质的方法。我们测试了基于质粒重组的基因改组的替代方案，发现枯草芽孢杆菌可以有效地重组具有 4% 分歧的序列，而大肠杆菌 mutS 更适合具有 22% 分歧的序列。
Gene shuffling is a way of creating proteins with interesting new characteristics, starting from diverged sequences. We tested an alternative to gene shuffling based on plasmid recombination and found that Bacillus subtilis efficiently recombines sequences with 4% divergence, and Escherichia coli mutS is more appropriate for sequences with 22% divergence.