Involvement of ionic interactions in cytokine adsorption of polyethyleneimine-coated polyacrylonitrile and polymethyl methacrylate membranes in vitro
Involvement of ionic interactions in cytokine adsorption of polyethyleneimine-coated polyacrylonitrile and polymethyl methacrylate membranes in vitro
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DOI：
10.1007/s10047-020-01173-0
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发表时间：
2020-05-11
影响因子：
1.3
通讯作者：
Nishida, Osamu
中科院分区：
文献类型：
--
作者：
Moriyama, Kazuhiro;Kato, Yu;Nishida, Osamu
聚乙烯亚胺涂层聚丙烯腈（AN 69 ST）和聚甲基丙烯酸甲酯（PMMA）膜是有效的尼古丁吸附血液滤过器;然而，尼古丁吸附机制仍然难以捉摸。本研究调查了离子相互作用在细胞因子吸附到带负电荷的AN 69 ST膜和带中性电荷的PMMA膜的参与。使用AN 69 ST和PMMA血液滤过器在闭环循环系统中进行了30 min的实验性血液滤过。在基线以及血液滤过10 min和30 min时测量供试品溶液中的肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6和IL-8浓度。为了研究离子相互作用在细胞因子吸附中的作用，在血液滤过10 min时计算细胞因子清除率（CL），并使用不同pH水平（7.6、7.2和6.8）的三种溶液。AN 69 ST血液滤过期间，TNF-α、IL-6和IL-8的CL分别为38 +/- 6 mL/min、23 +/- 7 mL/min和78 +/- 3 mL/min，表明与其各自的等电点相关。在PMMA血液滤过期间，IL-6的CL峰值为31 +/- 76 mL/min，CL与等电点之间未观察到相关性。当供试品溶液的pH值从7.6变为6.8时，AN 69 ST血液滤过器中TNF-α、IL-6和IL-8的CL增加;而PMMA血液滤过器中未观察到此类趋势。这些结果表明，离子相互作用在AN 69 ST膜而非PMMA膜的细胞因子吸附中发挥作用，并突出了该发现的临床相关性以及进一步血液滤过器设计的潜在实际应用。
Polyethyleneimine-coated polyacrylonitrile (AN69ST) and polymethyl methacrylate (PMMA) membranes are effective cytokine-adsorbing hemofilters; however, the cytokine-adsorption mechanism remains elusive. This study investigated the involvement of ionic interactions in cytokine adsorption to a negatively charged AN69ST membrane and neutral-charged PMMA membrane. Experimental hemofiltration was performed for 30 min in a closed-loop circulation system using AN69ST and PMMA hemofilters. Tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-8 concentrations in the test solutions were measured at baseline and at 10 min and 30 min into hemofiltration. To investigate the involvement of ionic interactions in cytokine adsorption, cytokine clearance (CL) was calculated at 10 min into hemofiltration and with three types of solutions at various pH levels (7.6, 7.2, and 6.8). During AN69ST hemofiltration, the CLs of TNF-alpha, IL-6, and IL-8 were 38 +/- 6 mL/min, 23 +/- 7 mL/min, and 78 +/- 3 mL/min, respectively, demonstrating a relationship with their respective isoelectric points. During PMMA hemofiltration, the CL of IL-6 peaked at 31 +/- 76 mL/min, with no relationship observed between the CL and isoelectric point. When the pH of the test solution shifted from 7.6 to 6.8, the CLs of TNF-alpha, IL6, and IL-8 increased in the AN69ST hemofilter; whereas, no such trend was observed in the PMMA hemofilter. These results indicated that Ionic interactions play a role in cytokine adsorption by the AN69ST membrane but not the PMMA membrane and highlight the clinical relevance of this finding, as well as the potential practical applications for further hemofilter design.