Chondrogenesis in a cell-polymer-bioreactor system
Chondrogenesis in a cell-polymer-bioreactor system
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DOI：
10.1006/excr.1998.4010
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发表时间：
1998-04-10
影响因子：
3.7
通讯作者：
Vunjak-Novakovic, G
中科院分区：
文献类型：
--
作者：
Freed, LE;Hollander, AP;Vunjak-Novakovic, G
软骨发生进行了研究，在体外控制条件下，使用细胞聚合物生物反应器系统。将小牛关节软骨细胞接种在可生物降解的聚合物支架上，并在旋转的生物反应器容器中培养。糖胺聚糖（GAG）和II型胶原蛋白的量的伴随增加导致在培养40天后在其整个横截面（6.7mm直径X 5 mm厚）上具有连续软骨基质的细胞-聚合物构建体。与天然小牛软骨相比，构建体具有相当的细胞度，每克湿重68%的GAG和33%的II型胶原蛋白。软骨细胞-聚合物构建体中软骨形成的进展与先前提出的体外前体细胞和体内肢体发育相似。特别是，聚合物支架提供了一种三维结构，可以用软骨细胞以高细胞密度接种，以建立细胞与细胞的合同，并启动软骨组织发育，而生物反应器容器提供了一个允许的微环境软骨发生。这项工作表明，使用组织工程构建体的细胞相互作用和分化的体外研究的承诺。(C)北京：科学出版社.
Chondrogenesis was studied under controlled in vitro conditions using a cell-polymer-bioreactor system. Bovine calf articular chondrocytes were seeded onto biodegradable polymer scaffolds and cultured in rotating bioreactor vessels. Concomitant increases in the amounts of glycosaminoglycan (GAG) and type II collagen resulted in cell-Polymer constructs with continuous cartilaginous matrix over their entire cross sections (6.7 mm diameter x 5 mm thick) after 40 days of cultivation. As compared to natural calf cartilage, constructs had comparable cellularities, 68% as much GAG and 33% as much type II collagen per gram wet weight, The progression of chondrogenesis in chondrocyte-polymer constructs was similar to that suggested previously for precursor cells in vitro and developing limbs in vivo. In particular, the polymer scaffold provided a three-dimensional structure that could be seeded with chondrocytes at high cell densities in order to establish cell-to-cell contracts and initiate cartilage tissue development, whereas the bioreactor vessel provided a permissive microenvironment for chondrogenesis. This work demonstrates the promise of using tissue engineered constructs for in vitro studies of cell interactions and differentiation. (C) 1998 Academic Press.