THE ROLE OF CIS-ACTING PROMOTER ELEMENTS IN TISSUE-SPECIFIC ALBUMIN GENE-EXPRESSION
THE ROLE OF CIS-ACTING PROMOTER ELEMENTS IN TISSUE-SPECIFIC ALBUMIN GENE-EXPRESSION
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DOI：
10.1126/science.2711183
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发表时间：
1989-04-21
期刊：
影响因子：
56.9
通讯作者：
SCHIBLER, U
中科院分区：
文献类型：
--
作者：
MAIRE, P;WUARIN, J;SCHIBLER, U
小鼠白蛋白基因启动子具有六个紧密间隔的核蛋白结合位点，位于TATA基序和核苷酸位置-170之间。用含有突变或聚合白蛋白启动子元件的模板的肝或脾核提取物进行体外转录，建立了组织特异性白蛋白基因转录的不同蛋白质结合位点的层次结构。HNF-1和C/EBP结合位点以组织特异性的方式强烈激活转录。NF-Y结合位点的激活电位较低，特异性较差，在肝脏和脾脏核提取物中同样有效。其余元素是相对较弱的激活位点。
The mouse albumin gene promoter has six closely spaced binding sites for nuclear proteins that are located between the TATA motif and nucleotide position -170. In vitro transcription with liver or spleen nuclear extracts of templates containing either mutated or polymerized albumin promoter elements establishes a hierarchy of the different protein binding sites for tissue-specific albumin gene transcription. The HNF-1 and C/EBP binding sites strongly activate transcription in a tissue-specific manner. The NF-Y binding site has a lower activation potential and is less specific, being equally efficient in liver and spleen nuclear extracts. The remaining elements are relatively weak activator sites.