Determination of intact-tissue glycerophosphorylcholine levels by quantitative 31P nuclear magnetic resonance spectroscopy and correlation with spectrophotometric quantification.
Determination of intact-tissue glycerophosphorylcholine levels by quantitative 31P nuclear magnetic resonance spectroscopy and correlation with spectrophotometric quantification.
复制标题
通过定量 31P 核磁共振波谱法测定完整组织甘油磷酸胆碱水平并与分光光度定量相关。
DOI：
10.1016/0003-2697(85)90116-2
复制
发表时间：
1985
影响因子：
2.9
通讯作者：
Gross,RW
中科院分区：
文献类型：
--
作者：
Billadello,JJ;Gard,JK;Ackerman,JJ;Gross,RW
本文建立了一种用~（31）P核磁共振光谱法动态定量完整组织中甘油磷酸胆碱（GPC）含量的方法，并用分光光度法进行了验证。使用外部标准对完整组织核磁共振区域进行定量，并校正磁化饱和度。通过洛伦兹线形分析和随后的与外标归一化的积分的交互式计算机化光谱拟合用于GPC浓度的绝对定量。血流动力学和代谢未受损的Langendorff灌注兔心脏含有1.70 ± 0.23 μmol GPC/g湿重。该值与采用甘油磷酰-[Me-3 H]胆碱作为内标物并采用分光光度法定量的分析技术测定的1.45 ± 0.23 μmol GPC/g湿重值无统计学显著差异。两种方法的标准误差分别为11%和10%。采用分光光度法测定内标物的回收率为95 ± 8%。这些方法的应用应有利于在几种疾病状态中注意到的甘油磷酸胆碱组织水平的变化的定量。
A method for the dynamic quantification of glycerophosphorylcholine (GPC) levels in intact tissue by31P nuclear magnetic resonance spectroscopy was developed and verified by a spectrophotometric technique. Intact tissue nuclear magnetic resonance areas were quantified utilizing an external standard and were corrected for magnetization saturation. Interactive computerized spectral fitting through Lorentzian lineshape analysis and subsequent integration with normalization to the external standard was utilized for the absolute quantification of GPC concentration. Hemodynamically and metabolically uncompromised Langendorff perfused rabbit hearts contained 1.70 ± 0.23 μmol GPC/g wet wt. This value was not statistically significantly different from the value of 1.45 ± 0.23 μmol GPC/g wet wt determined by an analytical technique employing glycerophosphoryl-[Me-3H]choline as an internal standard with spectrophotometric quantification. Both methods were accurate with a standard error of 11 and 10%, respectively. The recovery of internal standards utilizing the spectrophotometric technique was 95 ± 8%. The application of these methods should facilitate the quantification of changes in tissue levels of glycerophosphorylcholine noted in several disease states.