2‐Oxoglutarate increases the binding affinity of the NtcA (nitrogen control) transcription factor for the Synechococcus glnA promoter
2‐Oxoglutarate increases the binding affinity of the NtcA (nitrogen control) transcription factor for the Synechococcus glnA promoter
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DOI：
10.1016/s0014-5793(02)02219-6
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发表时间：
2002-02
期刊：
影响因子：
3.5
通讯作者：
M. F. Vázquez-Bermúdez;A. Herrero;E. Flores
中科院分区：
文献类型：
--
作者：
M. F. Vázquez-Bermúdez;A. Herrero;E. Flores
The cyanobacterial NtcA global nitrogen regulator belongs to the catabolite activator protein (CAP) family and activates transcription of nitrogen assimilation genes in response to nitrogen step‐down. The binding affinity of NtcA towards a DNA fragment carrying the promoter of theglnAgene fromSynechococcussp. PCC 7942, analyzed in vitro by band‐shift assay, was increased five‐fold by 2‐oxoglutarate in the presence of Mg2+ions. The 2‐oxoglutarate effect peaked at about 0.6 mM, a rather physiological concentration for this compound under nitrogen‐limiting conditions, and could be partially reproduced by 3‐oxoglutarate but not by oxaloacetate or glutamate. These results suggest 2‐oxoglutarate as a signal of the C to N balance of the cells to regulate NtcA activity and provide a new example of regulation in the versatile CAP family of proteins.
