Neutral growth inhibitors in light‐grown dwarf pea shoots –separation, identification and growth regulation
Neutral growth inhibitors in light‐grown dwarf pea shoots –separation, identification and growth regulation
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光生矮豌豆芽中的中性生长抑制剂——分离、鉴定和生长调节
DOI：
10.1111/j.1399-3054.1989.tb05455.x
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发表时间：
1989
影响因子：
6.4
通讯作者：
T. Hashimoto
中科院分区：
文献类型：
--
作者：
H. Noguchi;S. Tsurumi;Yasuyoshi Takeuchi;T. Hashimoto
为了将矮秆豌豆的内源生长抑制剂与其矮秆症联系起来，我们对矮秆豌豆（Pisum sativum L. cvs Progress No . 9和Alaska）光生苗的丙酮提取物的中性组分进行了全面的研究。从这两个品种中分离出6种抑制剂，并根据其在硅胶柱上的洗脱顺序命名为A-1、A-2、A-3、B-1、B-2和B-3。水芹根生物测定法测定其含量为：；进展0.40,16.5,6.36,1.02,0.11和0.10,cv。阿拉斯加0.33、2.35、3.51、0.95、0.10和0.09衣品单位（克鲜重）−1。将其对相关豌豆植株的生长调节贡献估计为上述含量乘以各标准样品在水芹根和相关豌豆品种中的比活性之比的乘积，分别为；在简历。进展A-2, 5.44 A-3, 2.10, cv。阿拉斯加A-2为0.68，A-3为0.88，其中A-2和A-3占6种抑制剂总贡献率的90%以上。A-2和A-3的含量和对生长的调节作用差异较大，表明A-2和A-3的含量越高，对这两种抑制剂的响应性越强。进展可能是该品种矮化的原因。a - 1和a -3与pisatin以及顺式、反式和反式、反式黄嘌呤的混合物进行了光谱鉴定。
To correlate endogenous growth inhibitors of dwarf pea to its dwarfism a thorough search of growth inhibitors was made in the neutral fraction of an acetone extract from the shoots of light-grown seedlings of Pisum sativum L. cvs Progress No 9 and Alaska. From both cultivars six inhibitors were separated and named A-1, A-2, A-3, B-1, B-2 and B-3 based on their order of elution from the silica gel column. Their contents as determined by cress root bioassay were: in cv. Progress 0.40, 16.5, 6.36, 1.02, 0.11 and 0.10, and in cv. Alaska 0.33, 2.35, 3.51, 0.95, 0.10 and 0.09 cress units (g fresh weight)−1. Their contributions to growth regulation of the relevant pea plants were estimated as the products of the above-stated contents times the ratios of the specific activities of each standard sample in the cress roots and the relevant pea cultivars, and they were; in cv. Progress A-2, 5.44 and A-3, 2.10, and in cv. Alaska A-2, 0.68 and A-3, 0.88, those of A-2 and A-3 constituting more than 90% of the total contribution of the six inhibitors in either cultivar. The great differences in the content and contribution to growth regulation of A-2 and A-3 between the two cultivars suggest that the higher contents of and greater responsiveness to the two inhibitors in cv. Progress may be causes of dwarfism of this cultivar. A-l and A-3 were spectroscopically identified with pisatin and a mixture of cis, trans–, and trans, trans xanthoxins.