Growth-Related Changes in Subcellular Ion Patterns in Maize Leaves (Zea mays L.) under Salt Stress
Growth-Related Changes in Subcellular Ion Patterns in Maize Leaves (Zea mays L.) under Salt Stress
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DOI：
10.1111/j.1439-037x.2011.00487.x
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发表时间：
2012-02
影响因子：
3.5
通讯作者：
M. Shahzad;K. Witzel;C. Zörb;Karl H. Mühling
中科院分区：
文献类型：
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作者：
M. Shahzad;K. Witzel;C. Zörb;Karl H. Mühling
Na +积累在叶质外体已被建议导致脱水，后来萎蔫，最后，死亡的受影响的叶片。我们的目的是评估敏感玉米的植物生长响应于盐的减少是否与叶质外体中较高量的Na +和Cl）浓度相关。采用去离子水渗透法研究了完整叶片中亚细胞离子的分布。我们发现，与对照相比，可溶性Na +和Cl）浓度分别增加了约16倍和4倍。这些浓度的特点扩展叶的质外体，完全在盐度下发展。与对照相比，盐胁迫下共质体中的K +浓度显著降低了64%.结果表明，盐胁迫下叶片中Ca 2+浓度显著降低，表明Ca 2+浓度与盐胁迫下叶片展度的降低有关。由于盐处理期间质外体Na +浓度的绝对增加远低于共质体Na +浓度的增加，因此玉米盐处理似乎不会导致由高质外体Na +浓度所施加的渗透胁迫，这已被建议用于其他植物物种（Oertli假说）。
Na + accumulation in the leaf apoplast has been suggested to lead to dehydration, later wilting and finally, the death of the affected leaves. Our aim has been to evaluate whether the reduction in the plant growth of sensitive maize in response to salinity is correlated with higher amounts of Na + and Cl ) concentrations in the leaf apoplast. Subcellular ion patterns in intact leaves were investigated by using deionised water infiltration. We found an increase in soluble Na + and Cl ) concentrations of about 16- and 4-fold, respectively, compared with the control. These concentrations characterized the apoplasts of expanding leaves that had entirely developed under salinity. Interestingly, the K + concentration was significantly reduced by 64 % compared with its control in the symplast under salinity. Our finding of a significantly decreased Ca 2+ concentration in shoots suggested a possible association of Ca 2+ concentration with the reduction in leaf expansion under salinity. As the absolute increase in the apoplastic Na + concentration during salt treatment was much lower compared with the increase in the symplastic Na + concentration, salt treatment in maize appears not to result in osmotic stress imposed by a high apoplastic Na + concentration as has been suggested for other plant species (Oertli hypothesis).