VASOPRESSINERGIC INNERVATION OF THE MOUSE SUPRACHIASMATIC NUCLEUS - AN IMMUNOELECTRON MICROSCOPIC ANALYSIS
VASOPRESSINERGIC INNERVATION OF THE MOUSE SUPRACHIASMATIC NUCLEUS - AN IMMUNOELECTRON MICROSCOPIC ANALYSIS
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DOI：
10.1002/cne.902980204
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发表时间：
1990-08-08
影响因子：
2.5
通讯作者：
HARARI, N
中科院分区：
文献类型：
--
作者：
CASTEL, M;FEINSTEIN, N;HARARI, N
人们正在尝试解开视交叉上核的局部电路，以期最终将特定的神经元系统与昼夜节律事件联系起来。因此，在光镜和电镜水平上对实验室小鼠该核的血管加压能神经支配进行了免疫细胞化学分析。单克隆抗神经生长素和多克隆抗血管加压素用于醛固定脑。制备相应前脑区域的连续振动体切片，进行包埋前免疫过氧化物酶染色和/或包埋后免疫金标记。整个视交叉上核均可见免疫反应性体、突起、静脉曲张和突触终末，其比例和密度在不同部位有所不同。抗利尿激素能体的主要类型为卵形至圆形（7-10 .mu）。M)，含有分泌颗粒（85-120 nm），其中大部分具有免疫反应性。轴突末端，无论是非免疫反应性的还是免疫反应性的，都影响着抗利尿激素能的躯体和过程，经常表现出突触特化。抗利尿激素能末端，含有分泌颗粒和微泡，遍布细胞核，特别是在背内侧神经节内。这些标记的终端大小不等（0.4-3.4 .mu）。M2)和形状，从紧凑的钮扣到多形性的轮廓，一些被突触后棘深深地凹进，树突或体细胞。约65%的抗利尿激素终端为轴突，30%为轴体细胞；约5%为轴突。至少四分之一的抗利尿激素突触末端为轴突。涉及抗利尿激素元件（体细胞、树突）的其他形式的神经元间接触包括广泛的膜到膜的附着位点和多个附着点。小鼠视交叉上核中含有抗利尿激素的神经元之间的多功能性相互连接表明，这是一个高度活跃和协调的网络，它在很大程度上有助于局部核内回路。此外，密集的血管加压能输出向背侧的下丘脑脑室周围输出，在那里可能与不同的下丘脑神经内分泌系统建立直接联系。
Attempts are being made to unravel the local circuitry of the suprachiasmatic nucleus, with a view toward eventually correlating specific neuronal systems with circadian events. Hence, the vasopressinergic innervation of this nucleus in the laboratory mouse has been analyzed immunocytochemically at the light and electron microscopical levels. Monoclonal antineurophysin and polyclonal antivasopressin were used on aldehyde-fixed brains. Serial vibratome sections of the appropriate forebrain region were prepared for pre-embedding immunoperoxidase staining and/or postembedding immunogold labeling. Immunoreactive somata, processes, varicosities, and synaptic terminals were found throughout the suprachiasmatic nucleus, their ratio and density varying at different locations. The predominant type of vasopressinergic soma was ovoid to rounded (7-10 .mu.m), containing secretory granules (85-120 nm), a large proportion of which were immunoreactive. Axon terminals, both nonimmunoreactive and immunoreactive, impinged upon vasopressinergic somata and processes, often displaying synaptic specializations. Vasopressinergic terminals, containing secretory granules and microvesicles, were found throughout the nucleus, particularly within the dorsomedial neuropil. These labeled terminals varied in size (0.4-3.4 .mu.m2) and shape, ranging from compact boutons to pleomorphic profiles, some deeply indented by postsynaptic spines, either dendritic or somatic. Approximately 65% of the vasopressin-containing terminals were axodendritic and 30% axosomatic; about 5% appeared to be axoaxonic. At least a quarter of all vasopressinergic synaptic terminals were axospinous. Other forms of interneuronal contact involving vasopressinergic elements (somata, dendrites) included extensive membrane to membrane appositional sites, and multiple puncta adhaerentia. The versatility of interconnections between vasopressin-containing neurons in the mouse suprachiasmatic nucleus suggests a highly active and coordinated network, which contributes substantially to local intranuclear circuitry. In addition, a dense efferent vasopressinergic output is directed dorsally towards the periventricular hypothalamus, where direct associations may be established with diverse hypothalamic neuroendocrine systems.