Circulating tumor cells with a putative stem cell phenotype in peripheral blood of patients with breast cancer
Circulating tumor cells with a putative stem cell phenotype in peripheral blood of patients with breast cancer
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DOI：
10.1016/j.canlet.2009.06.027
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发表时间：
2010-02-01
期刊：
影响因子：
9.7
通讯作者：
Georgoulias, Vassilis
中科院分区：
文献类型：
--
作者：
Theodoropoulos, Panayiotis A.;Polioudaki, Hara;Georgoulias, Vassilis
CD 44（+）/CD 24（-/低）和ALDH 1（+）细胞表型与乳腺癌的干性和增强的致瘤潜力相关。我们使用三标记免疫荧光显微镜评估了转移性乳腺癌患者外周血（CTC）中循环的肿瘤细胞CD 44、CD 24和ALDH 1的表达。在30名患者中的20名（66.7%）中鉴定的总共1439个CTC中，35.2%具有干细胞样/致瘤表型CD 44（+）/CD 24（-/低），而在7名患者中分析的17.7%的CTC为ALDH 1（高）/CD 24（-/低）。总之，我们报告了转移性乳腺癌患者中存在具有假定干细胞祖细胞表型的CTC亚群。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
The CD44(+)/CD24(-/low) and ALDH1(+) cell phenotypes are associated with sternness and enhanced tumorigenic potential in breast cancer. We assessed the expression of CD44, CD24 and ALDH1 on tumor cells circulating in the peripheral blood (CTCs) of patients with metastatic breast cancer using triple-marker immurrofluorescence microscopy. Among a total of 1439 CTCs identified in 20 (66.7%) out of 30 patients, 35.2% had the stem-like/tumorigenic phenotype CD44(+)/CD24(-/low), whereas 17.7% of the CTCs analyzed in seven patients, were ALDH1(high)/CD24(-/low). In conclusion, we report the existence of a subpopulation of CTCs with putative stem cell progenitor phenotypes in patients with metastatic breast cancer. (C) 2009 Elsevier Ireland Ltd. All rights reserved.