Blockade of T-Type Ca2+ Channels Inhibits Human Ovarian Cancer Cell Proliferation
Blockade of T-Type Ca2+ Channels Inhibits Human Ovarian Cancer Cell Proliferation
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DOI：
10.3109/07357907.2011.568565
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发表时间：
2011-06-01
影响因子：
2.4
通讯作者：
Li, Ming
中科院分区：
文献类型：
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作者：
Li, Wei;Zhang, Shu-Lan;Li, Ming
钙离子通道的调节与肿瘤细胞的发展密切相关。我们在此报道，与正常卵巢组织相比，人卵巢癌组织中T型钙通道的表达显著增加。用NNC55-0396、米贝法地尔阻断T型钙通道，或用siRNA-Ca(V)3.1/3.2特异性下调这些蛋白的表达，均可抑制两株卵巢癌细胞的增殖，增加细胞周期中G0/G1期的比例。此外，NNC55-0396可延缓裸鼠卵巢癌的形成。因此，T型钙通道的功能对人卵巢癌细胞的增殖具有重要意义。
Regulation of Ca2+ channels has been implicated in the progression of tumor cells. We report here that T-type Ca2+ channel expression in human ovarian cancer tissues is greatly increased compared to normal ovarian tissues. Blockade of T-type Ca2+ channel with NNC 55-0396, mibefradil, or by specifically knocking down the expression of these proteins with siRNA-Ca(v)3.1/3.2 suppressed the proliferation of two ovarian cancer cell lines and increased G0/G1 phase distribution in the cell cycle. Furthermore, NNC 55-0396 slowed ovarian cancer formation in nudemice. Therefore the function of T-type Ca2+ channels is important for the proliferation of human ovarian cancer cells.