Prevalence and genetic diversity of Aichi virus strains in stool samples from community and hospitalized patients
Prevalence and genetic diversity of Aichi virus strains in stool samples from community and hospitalized patients
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DOI：
10.1128/jcm.02140-07
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发表时间：
2008-04-01
影响因子：
9.4
通讯作者：
Pothier, P.
中科院分区：
文献类型：
--
作者：
Ambert-Balay, K.;Lorrot, M.;Pothier, P.
爱知病毒已被认为是胃肠炎的病原体。通过对 3C 和 3D (3CD) 非结构蛋白基因组区域进行逆转录 PCR (RT-PCR) 扩增，筛选了因急性腹泻住院的儿童的总共 457 份粪便样本以及参与 110 次胃肠炎爆发的成人和儿童的 566 份粪便样本中是否存在爱知病毒。我们的结果显示，儿科样本中爱知病毒的发病率较低，并且在某些情况下存在与其他微生物病原体的混合感染。从疫情调查来看，爱知病毒的存在似乎是导致胃肠炎爆发的混合感染的一个指标，并且它可能涉及一半与牡蛎相关的疫情。开发了第二种 RT-PCR 来扩增 VP1 基因的一部分。系统发育分析显示，基于 3CD 和 VP1 基因序列的两个分类之间具有良好的相关性，并揭示了 A 基因型在法国的流行情况。它还使我们能够部分描述可以代表新基因型的爱知病毒株，从而表明存在一定的多样性。
Aichi virus has been proposed as a causative agent of gastroenteritis. A total of 457 stool specimens from children hospitalized with acute diarrhea and 566 stool specimens from adults and children involved in 110 gastroenteritis outbreaks were screened for the presence of Aichi virus by reverse transcription-PCR (RT-PCR) amplification of the genomic region of the 3C and 3D (3CD) nonstructural proteins. Our results show a low incidence of Aichi virus in pediatric samples and the existence of mixed infections with other microbiological agents in some cases. From the outbreak survey, it appears that the presence of Aichi virus is an indicator of mixed infections causing gastroenteritis outbreaks and that it could be involved in half of the oyster-associated outbreaks. A second RT-PCR was developed to amplify a part of the VP1 gene. The phylogenetic analysis showed a good correlation between the two classifications based on 3CD and VP1 gene sequences and revealed the prevalence of genotype A in France. It also allowed us to partially describe an Aichi virus strain that could represent a new genotype, thus suggesting the existence of a certain diversity.