Characterization of the genomic structure and promoter of the mouse NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase gene
Characterization of the genomic structure and promoter of the mouse NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase gene
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DOI：
10.1006/bbrc.1997.6831
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发表时间：
1997-06-27
影响因子：
3.1
通讯作者：
Tai, HH
中科院分区：
文献类型：
--
作者：
Matsuo, M;Ensor, CM;Tai, HH
从129个小鼠ES噬菌体P1基因组文库中克隆了小鼠NAD（+）依赖的15-羟基前列腺素脱氢酶（15-PGDH）基因及其5 ′侧翼区，该基因含有7个外显子和6个内含子，全长11.3kb。转录起始位点位于ATG起始密码子上游35个碱基处。1.6kb启动子区的核苷酸序列包含两个TATA盒和许多潜在的调控元件，包括Sp1、CRE、GRE、AP 1、AP 2、NF-IL 6和雌激素受体结合位点。启动子活性的研究表明，在用佛波醇酯刺激后，5 '侧翼区的前400个核苷酸有效地驱动U936细胞中荧光素酶报告基因的转录。(C)1997年学术出版社。
The mouse NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) gene and its 5'-flanking region was cloned from a 129 mouse ES bacteriophage P1 genomic library, The gene contains 7 exons and 6 introns and is 11.3 kb in length. The transcription initiation site was mapped at 35 bases upstream from the ATG start codon. The nucleotide sequence of the 1.6 kb promoter region contains two TATA boxes and a number of potential regulatory elements including Sp1, CRE, GRE, AP1, AP2, NF-IL6 and estrogen receptor binding site. Studies of the promoter's activity showed that the first 400 nucleotides of 5'-flanking region efficiently drove the transcription of the luciferase reporter gene in U936 cells upon stimulation with a phorbol ester. (C) 1997 Academic Press.