The voltage-dependent sodium channel in mammalian CNS and PNS: antibody characterization and immunocytochemical localization.
The voltage-dependent sodium channel in mammalian CNS and PNS: antibody characterization and immunocytochemical localization.
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哺乳动物中枢神经系统和三七总皂苷中的电压依赖性钠通道：抗体表征和免疫细胞化学定位。
DOI：
10.1016/0006-8993(90)91763-7
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Angelides,KJ
中科院分区：
文献类型：
--
作者：
Elmer,LW;Black,JA;Waxman,SG;Angelides,KJ
Monoclonal and polyclonal antibodies were generated against the voltage-dependent sodium channel purified from rat brain, and were used to characterize and localize sodium channels within mammalian central nervous system (CNS) and peripheral nervous system (PNS). These antibodies immunoblot and immunoprecipitate from labeled membrane proteins a 260-kDa polypeptide, as well as immunoprecipitate sodium channels saturated with [3H]saxitoxin. These monoclonal and polyclonal antibodies do not, however, recognize sodium channels in cardiac or skeletal muscle. Immunocytochemical analyses of cultured CNS and PNS neurons and immuno-ultrastructural localization of sodium channel reactivity within CNS tissue in situ indicate that these probes provide a unique tool for studying the level of expression, organization and turnover of sodium channels within the CNS and PNS.
