One of the two trout proopiomelanocortin messenger RNAs potentially encodes new peptides.
One of the two trout proopiomelanocortin messenger RNAs potentially encodes new peptides.
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鳟鱼的两种原阿黑皮质素信使 RNA 之一可能编码新的肽。
DOI：
10.1210/mend.6.10.1448114
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发表时间：
1992
影响因子：
--
通讯作者：
P. Jego
中科院分区：
文献类型：
--
作者：
G. Salbert;I. Chauveau;G. Bonnec;Y. Valotaire;P. Jego
POMC是许多生物活性肽如ACTH、α-MSH、β-MSH和β-内啡肽的前体。众所周知，这些肽中的一些，特别是β-内啡肽，参与调节哺乳动物的生殖功能。为了研究POMC衍生肽在控制鱼类繁殖中的可能作用，我们克隆并测序了两种不同的鳟鱼POMC cDNA，称为POMC A和POMC B。这些cDNA表现出有限的序列同源性（44%）。推导的氨基酸序列也显示出弱的相似性（43%），尽管一些肽序列（α-MSH，β-MSH和β-内啡肽）的高度保守性。POMC A编码序列显示出不寻常的长度，产生了有史以来最长的内啡肽序列。β-内啡肽A的长羧基末端部分含有三个潜在的二元切割位点，允许三个新肽的出现：EQWGREEGEE，ALGE和YHFQG。利用原位杂交技术，我们发现这两个POMC基因在同一垂体细胞中表达。POMC A mRNA是唯一一个可检测到的下丘脑性活跃的鱼，而两个POMC基因表达在下丘脑性活跃的鱼。这些结果表明，两个功能POMC基因存在于虹鳟鱼。在POMC神经元中，POMC B基因的表达可能受性类固醇激素的控制。
POMC is the precursor for a number of biologically active peptides such as ACTH, alpha-MSH, beta-MSH, and beta-endorphin. It is well known that some of these peptides, especially beta-endorphin, are involved in the regulation of reproductive functions in mammals. In order to investigate the possible role of POMC-derived peptides in the control of fish reproduction, we have cloned and sequenced two different trout POMC cDNAs called POMC A and POMC B. These cDNAs exhibited limited sequence homology (44%). The deduced amino acid sequences also showed weak similarity (43%), despite the high conservation of some peptide sequences (alpha-MSH, beta-MSH, and beta-endorphin). The POMC A coding sequence exhibited an unusual length, generating the longest endorphin ever sequenced. The long carboxy-terminal part of the beta-endorphin A contained three potential dibasic cleavage sites, allowing the occurrence of three new peptides: EQWGREEGEE, ALGE, and YHFQG. Using in situ hybridization, we found that the two POMC genes were expressed in the same pituitary cells. POMC A mRNA was the only one detectable in the hypothalamus of sexually inactive fish, whereas the two POMC genes were expressed in the hypothalamus of sexually active fish. These results indicate that two functional POMC genes are present in the rainbow trout. In POMC neurons, the expression of the POMC B gene is likely to be under the control of sexual steroids.
影响因子：
2.7
作者：
COX, KH;DELEON, DV;ANGERER, RC
通讯作者：
ANGERER, RC