Model-based analysis of clinical fluorescence spectroscopy for in vivo detection of cervical intraepithelial dysplasia
Model-based analysis of clinical fluorescence spectroscopy for in vivo detection of cervical intraepithelial dysplasia
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DOI：
10.1117/1.2187979
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发表时间：
2006-03-01
影响因子：
3.5
通讯作者：
Richards-Kortum, Rebecca
中科院分区：
文献类型：
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作者：
Chang, Sung K.;Marin, Nena;Richards-Kortum, Rebecca
我们提出了一个数学模型来计算上皮和基质中光散射体、光吸收体和荧光团的相对浓度。该数学描述迭代地拟合到体内测量的荧光光谱，产生每个分子的相对浓度。该数学模型被应用到从292例患者体内获得的正常和发育异常的宫颈组织的总共493个荧光测量。将估计的参数与组织病理学诊断进行比较，以评估其诊断潜力。使用已知的光学参数组模拟的荧光光谱的数学模型进行验证。随后将数学模型应用于来自宫颈组织的体内荧光测量，产生精确描述测量数据的拟合。从493个荧光测量估计的光学参数显示与正常组织相比，异型增生组织中上皮黄素腺嘌呤二核苷酸（FAD）荧光增加，上皮角蛋白荧光减少，上皮光散射增加，基质胶原荧光减少，以及基质血红蛋白光吸收增加。这些变化可能反映了代谢活性的增加和上皮异型增生细胞的分化丧失，以及与异型增生相关的基质血管生成。这里提出的模型提供了一种工具，分析临床荧光光谱产生的定量信息有关的分子变化发育异常转化。(c)2006年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。
We present a mathematical model to calculate the relative concentration of light scatterers, light absorbers, and fluorophores in the epithelium and stroma. This mathematical description is iteratively fit to the fluorescence spectra measured in vivo, yielding relative concentrations of each molecule. The mathematical model is applied to a total of 493 fluorescence measurements of normal and dysplastic cervical tissue acquired in vivo from 292 patients. The estimated parameters are compared with histopathologic diagnosis to evaluate their diagnostic potential. The mathematical model is validated using fluorescence spectra simulated with known sets of optical parameters. Subsequent application of the mathematical model to in vivo fluorescence measurements from cervical tissue yields fits that accurately describe measured data. The optical parameters estimated from 493 fluorescence measurements show an increase in epithelial flavin adenine dinucleotide (FAD) fluorescence, a decrease in epithelial keratin fluorescence, an increase in epithelial light scattering, a decrease in stromal collagen fluorescence, and an increase in stromal hemoglobin light absorption in dysplastic tissue compared to normal tissue. These changes likely reflect an increase in the metabolic activity and loss of differentiation of epithelial dysplastic cells, and stromal angiogenesis associated with dysplasia. The model presented here provides a tool to analyze clinical fluorescence spectra yielding quantitative information about molecular changes related to dysplastic transformation. (c) 2006 Society of Photo-Optical Instrumentation Engineers.