Response surface methodology for optimizing the fermentation medium of Clostridium butyricum
Response surface methodology for optimizing the fermentation medium of Clostridium butyricum
复制标题
DOI：
10.1111/j.1472-765x.2004.01595.x
复制
发表时间：
2004-01-01
影响因子：
2.4
通讯作者：
Ding, LX
中科院分区：
文献类型：
--
作者：
He, GQ;Kong, Q;Ding, LX
目的：酪酸梭菌菌株已越来越多地被用作动物和人类的益生菌。方法与结果：采用响应曲面法(RSM)分析了葡萄糖、果胶、豆饼浸出物、酪蛋白、玉米浆、硫酸铵、碳酸氢钠浓度、初始pH等因素对培养效果的影响。采用部分析因设计对本实验室保存的一株丁酸菌益生菌生长的主要影响因素进行了研究，并采用中心复合试验设计建立了优化发酵培养基组成的统计模型。结果表明，丁酸乳杆菌的最佳发酵条件为：葡萄糖2%(w/v)，果胶0.5%，酪蛋白0.2%，豆饼浸膏3.98%，硫酸铵0.1%，碳酸氢钠0.124%，玉米浆0.37%，硫酸锰0.02%。0.02%硫酸镁7H(2)O(w/v)，0.002%CaCl2(w/v)，pH 7.5。结论：在最佳发酵条件下培养24 h，活菌数估计为10(9)cfu ml(-1)。在这项研究中，我们报告了利用统计方法优化产生增加产量的生长介质。研究的意义和影响：细菌作为益生菌的使用正在显示出越来越大的潜力。开发高产量的生长介质显然是一种需要，而优化生长介质的方法是创新的。
Aims: Strains of Clostridium butyricum have been increasingly used as probiotics for both animals and humans. The aim of this study was to develop a growth medium for cultivating C. butyricum ZJUCB using a statistical methodology.Methods and Results: Response surface methodology (RSM) was used to evaluate the effects of variables, namely the concentrations of the glucose, pectin, soyabean cake extract, casein, corn steep flour, ammonium sulphate, sodium bicarbonate and the medium initial pH. A fractional factorial design was applied to study the main factors that affected the growth of a probiotic strain of C. butyricum currently preserved in our lab and the central composite experimental design was adopted to derive a statistical model for optimizing the composition of the fermentation medium. The experimental results showed that the optimum fermentation medium for the growth of C. butyricum was composed of 2% glucose (w/v), 0.5% pectin (w/v), 0.2% casein (w/v), 3.98% soyabean cake extract, 0.1% (NH4)(2)SO4 (w/v), 0.124% NaHCO3 (w/v), 0.37% corn steep flour (w/v), 0.02% MnSO4 H2O (w/v), 0.02% MgSO4 7H(2)O (w/v) and 0.002% CaCl2 (w/v) at pH 7.5.Conclusions: After incubating 24 h in the optimum fermentation medium, the populations of the viable organisms were estimated to be 10(9) CFU ml(-1). In the present study, we report the optimization of a growth medium that produced increased yields using statistical approach.Significance and Impact of the Study: The use of bacteria as a probiotic is showing increasing potential. The development of a growth medium that has a high yield is an obvious need, and the approach to optimizing a growth medium is innovative.