pH-sensitive cyanine dyes for biological applications
pH-sensitive cyanine dyes for biological applications
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DOI：
10.1023/a:1021366010681
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发表时间：
2002-12-01
影响因子：
2.7
通讯作者：
Kalinka, S
中科院分区：
文献类型：
--
作者：
Cooper, ME;Gregory, S;Kalinka, S
设计并合成了一系列功能化、水溶性、pH敏感的五次甲基花青(Cy(TM)5)染料。这些探针在酸性介质中发出荧光，但在碱性环境中不发出荧光。这些探针结构的细微变化可能会导致这些探针的 pKa 发生显着变化。这些探针已在细胞环境中使用 IN Cell 分析仪（一种用于基于细胞的测定成像的共焦成像仪）来检测 PH 的局部变化。
A series of functionalized, water-soluble, pH-sensitive pentamethine cyanine (Cy(TM)5) dyes has been designed and synthesized. These probes are fluorescent in acidic media but are non-fluorescent in an alkaline environment. Subtle changes to the structure of these probes can lead to pronounced changes in the pKa of these probes. These probes have been utilized in a cellular environment to detect localized changes in PH using the IN Cell Analyzer, a confocal imager formatted for imaging of cell-based assays.