cDNA cloning of a quail homeobox gene and its expression in neural crest-derived mesenchyme and lateral plate mesoderm.
cDNA cloning of a quail homeobox gene and its expression in neural crest-derived mesenchyme and lateral plate mesoderm.
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鹌鹑同源盒基因的 cDNA 克隆及其在神经嵴衍生间充质和侧板中胚层中的表达。
DOI：
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发表时间：
1990
影响因子：
11.1
通讯作者：
N. L. Le Douarin
中科院分区：
文献类型：
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作者：
Y. Takahashi;N. L. Le Douarin
我们在此报道克隆了与果蝇基因msh和小鼠基因Hox-7和Hox-7.1相关的鹌鹑cDNA。因此我们称其为cDNA Quox-7。同源盒及其5′和3′侧序列与上述基因的氨基酸同源性较高（83%），同源盒上游的另一段氨基酸同源性中等（43%）；在其他地方，鹌鹑和老鼠的cdna序列有明显的差异。在第2-5天的鹌鹑胚胎中，quax -7转录本主要存在于腹侧间质（面部和鳃裂下结构、躯体胸膜和四肢的神经嵴衍生的中胚层）以及浅层外胚层和神经管的狭窄背内侧细胞带中。这一模式基本上与报道的Hox-7/7.1相似，这表明这些基因的产物在不同种类脊椎动物的发育中起着相似的作用。
We report here the cloning of a quail cDNA related to the Drosophila gene msh and to the mouse genes Hox-7 and Hox-7.1. For this reason we called this cDNA Quox-7. The amino acid homology of Quox-7 cDNA with the above mentioned genes is high (83%) for the homeobox and its 5'- and 3'-flanking sequences, and the homology is medium (43%) for another stretch of amino acids upstream of the homeobox; elsewhere the sequences of quail and mouse cDNAs have diverged significantly. In quail embryos of day 2-5, Quox-7 transcripts were found essentially in the ventral mesenchyme (neural crest-derived mesectoderm of the face and hypobranchial structures, somatopleure, and limbs) and also in a narrow dorsomedial band of cells of the superficial ectoderm and neural tube. This pattern is fundamentally similar to that reported for Hox-7/7.1, suggesting that the products of these genes play a similar role in the development of the different classes of vertebrates.