Regional variation in the oxygenation of mouse mammary tumours in vivo demonstrated by fluorescence of pyridine nucleotide.
Regional variation in the oxygenation of mouse mammary tumours in vivo demonstrated by fluorescence of pyridine nucleotide.
复制标题
通过吡啶核苷酸的荧光证明体内小鼠乳腺肿瘤氧合的区域变化。
DOI：
--
复制
发表时间：
1972
影响因子：
2.6
通讯作者：
H. Reinhold
中科院分区：
文献类型：
--
作者：
M. Gosálvez;R. Thurman;B. Chance;H. Reinhold
摘要在小鼠皮肤腔中移植的乳腺肿瘤表面不同点，检测了细胞内还原型吡啶核苷酸的荧光。肿瘤荧光的变化是根据动物是否呼吸正常或降低浓度的氧气来记录的。缺氧时荧光增加代表所有血管化良好点有氧荧光的75- 100%。在血管化不良的肿瘤点或在那些邻近坏死中心的荧光增加缺氧是最小的。所述结果直接证实了小鼠乳腺肿瘤中缺氧区的存在。这些区域可能是由肿瘤毛细血管循环缺陷引起的。
Abstract Fluorescence due to intracellular reduced pyridine nucleotide has been examined in vivo at different points at the surface of mammary tumours transplanted in a skin chamber in mice. Changes in the tumour fluorescence were registered according to whether animals were breathing oxygen at normal or at reduced concentrations. The fluorescence increase in hypoxia represents 75–100 per cent of the aerobic fluorescence at all well-vascularized points. At poorly vascularized tumour points or at those adjacent to necrotic centres the fluorescence increase in hypoxia was minimal. The results described directly corroborate the existence of hypoxic areas in mammary tumours of the mouse. These areas were probably caused by defects in the capillary circulation of the tumour.