Identification and primary genetic analysis of Arabidopsis stomatal mutants in response to multiple stresses

Identification and primary genetic analysis of Arabidopsis stomatal mutants in response to multiple stresses
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拟南芥气孔突变体响应多重胁迫的鉴定和初步遗传分析

DOI:
10.1007/s11434-006-2173-2
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发表时间:
2006-11
期刊:
科学通报(英文版)
影响因子:
--
通讯作者:
Wang Pengtao
Wang Pengtao
中科院分区:
其他
文献类型:
--
作者:
Liu Hao;Song Yuwei;Zhou Yun;Miao Chen;Kang Yanli;An Guoyong;Song Chunpeng;Zhao Xiaoliang;Wang Pengtao

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为了应对多变的环境条件,位于叶表皮的保卫细胞可以整合并应对多种复杂的刺激,从而使气孔处于适当的状态。然而,保卫细胞信号传导中的许多信号成分仍然难以捉摸。在我们的实验室中,使用非侵入性远程红外热图像工具来筛选甲磺酸乙酯诱变群体中的拟南芥气孔反应突变体在多种胁迫(ABA、H2O2、CO2 等)下的情况。分离出四十多个“热”或“冷”突变体(与正常植株相比,温度高于或低于 0.5°C)。这些突变体的鉴定和初步遗传分析表明,它们是单基因隐性突变,与野生型相比,气孔孔径存在明显差异。这些突变体对各种环境应激和激素的反应进行了全面的研究,这使我们能够进一步了解不同信号转导途径中的串扰。
In response to variable environmental conditions, guard cells located in the leaf epidermis can integrate and cope with a multitude of complicated stimuli, thereby making stomata in an appropriate state. However, many signaling components in guard cell signaling remain elusive. In our laboratory, a tool for non-invasive remote infrared thermal images was used to screen an ethyl methane sulfonate-mutagenized population for Arabidopsis stomatal response mutants under multiple stresses (ABA, H2O2, CO2, etc.). More than forty “hot” or “cold” mutants were isolated (above or below 0.5°C in contrast to normal plantlets). Identification and primary genetic analysis of these mutants show that they are monogenic recessive mutations and there exist distinct difference in stomata apertures compared to wild type. These mutants in response to various environmental stresses and hormones were comprehensively investigated, which enables us to further understand the cross-talk in different signal transduction pathways.
DOI: 10.2307/2656643
发表时间: 2000-08-01
影响因子: 3
作者:
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