The Current Hepatitis C Virus Prevalence in China May Have Resulted Mainly from an Officially Encouraged Plasma Campaign in the 1990s: a Coalescence Inference with Genetic Sequences
The Current Hepatitis C Virus Prevalence in China May Have Resulted Mainly from an Officially Encouraged Plasma Campaign in the 1990s: a Coalescence Inference with Genetic Sequences
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DOI：
10.1128/jvi.01773-13
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发表时间：
2013-11-01
影响因子：
5.4
通讯作者：
Chen, Guihua
中科院分区：
文献类型：
--
作者：
Lu, Ling;Tong, Wangxia;Chen, Guihua
在这项研究中，我们研究了丙型肝炎病毒（HCV）的分子流行病学和进化动力学。在中国南方的433例患者中，379例同时具有E1和NS 5 B序列，并分为5种主要亚型：1b型256例，6a型67例，2a型29例，3a型14例，3b型13例。利用所获得的E1序列，沿着与来自中国样本的其他研究的序列，我们通过合并策略推断HCV流行历史。估计了5种亚型的5个贝叶斯天际线图（BSP）。他们同时强调，从1993年到2000年，HCV感染人口规模迅速增长，随后突然放缓。虽然两侧的人口规模不同，但图显示了HCV快速增长的独特模式。巧合的是，在1993年至2000年期间，由于官方鼓励的血浆活动中的程序错误，受污染的输血在中国很常见。1998年至2000年的突然放缓与1998年中央政府宣布有偿献血为非法相对应。使用参数模型，估计1993年至2000年期间HCV的人口增长率。结果表明，6a型的发生率最高，其次为1b型、2a型、3b型和3a型。由于这些比率彼此之间存在显著差异（P <1 e- 9），它们可能有助于解释为什么6a在中国南方越来越普遍，而1b在全国范围内占主导地位。这些比率比其他地方报告的比率高出约10倍。这些发现表明，在血浆活动期间，有效的病毒传播的某些障碍被清除，使HCV广泛传播。
In this study, we investigated hepatitis C virus (HCV) molecular epidemiology and evolutionary dynamics. Both E1 and NS5B sequences were characterized in 379 of 433 patients in southern China and classified into five major subtypes: 1b in 256 patients, 6a in 67 patients, 2a in 29 patients, 3a in 14 patients, and 3b in 13 patients. Using the E1 sequences obtained, along with those from other studies using samples from China, we inferred the HCV epidemic history by means of coalescence strategies. Five Bayesian skyline plots (BSPs) were estimated for the five subtypes. They concurrently highlighted the rapid growth in the HCV-infected population size from 1993 to 2000, followed by an abrupt slowing. Although flanked on both sides by variable population sizes, the plots showed distinct patterns of rapid HCV growth. Coincidently, 1993 to 2000 was a period when contaminated blood transfusions were common in China due to a procedural error in an officially encouraged plasma campaign. The abrupt slowing in 1998 to 2000 corresponded to the central government outlawing paid blood donations in 1998. Using a parametric model, the HCV population growth rates were estimated during 1993 to 2000. It was revealed that the 6a rate was the highest, followed by those of 1b, 2a, 3b, and 3a. Because these rates differed significantly (P < 1e - 9) from each other, they may help explain why 6a is increasingly prevalent in southern China and 1b is predominant nationwide. These rates are approximately 10-fold higher than those reported elsewhere. These findings suggested that during the plasma campaign, certain barriers to efficient viral transmission were removed, allowing wide HCV dissemination.