Heparin, lipoproteins, and oxygenated fatty acids in blood: A cautionary note
Heparin, lipoproteins, and oxygenated fatty acids in blood: A cautionary note
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DOI：
10.1016/j.plefa.2007.10.012
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发表时间：
2007-11-01
影响因子：
3
通讯作者：
Vollmer, A.
中科院分区：
文献类型：
--
作者：
Goodfriend, T. L.;Pedersen, T. L.;Vollmer, A.
我们测量了16个非酯化含氧脂肪酸衍生物（氧化脂质）从7个人类受试者的血浆。分析每例受试者的两份动脉样本，间隔约2小时。我们观察到第二个样品中大多数氧化脂质的水平显著增加，高达470倍。在第一份和第二份样本之间，受试者接受了约800-1000 IU的肝素，以防止血管内导管凝血。我们假设肝素激活脂蛋白脂肪酶，这反过来，释放氧化脂质从甘油三酯和磷脂在血浆脂蛋白。其中一些脂肪分解可能发生在样本储存期间。如果受试者在采血前给予肝素，以及如果储存样本中遗漏脂肪酶抑制剂，则人血浆中非酯化脂质的测量可能会失真。爱思唯尔有限公司出版
We measured 16 nonesterified oxygenated fatty acid derivatives (oxylipids) in plasmas from seven human subjects. Two arterial samples from each subject were analyzed, drawn approximately 2 h apart. We observed a marked increase in levels of most oxylipids in the second sample, as high as 470-fold. Between the first and second samples, subjects received approximately 800-1000 IU of heparin to prevent clotting in intravascular catheters. We postulate that heparin activated lipoprotein lipases, which, in turn, released oxylipids from triglycerides and phospholipids in plasma lipoproteins. Some of that lipolysis may have occurred during sample storage. Measurements of nonesterified lipids in human plasma may be distorted if heparin is administered to subjects before blood is drawn and if lipase inhibitors are omitted from stored samples. Published by Elsevier Ltd.