Gustatory responses of neurons in the nucleus of the solitary tract of behaving rats.
Gustatory responses of neurons in the nucleus of the solitary tract of behaving rats.
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行为大鼠孤束核神经元的味觉反应。
DOI：
10.1152/jn.1991.66.4.1232
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发表时间：
1991
影响因子：
2.5
通讯作者：
Norgren,R
中科院分区：
文献类型：
--
作者：
Nakamura,K;Norgren,R
1. 117个单神经元的活动记录在孤束核（NST）和测试与四个标准的化学刺激[蔗糖，氯化钠，柠檬酸，盐酸奎宁（QHCl）]和蒸馏水在清醒的，行为大鼠。在这些神经元中的101个中，至少有一个sapid刺激引起了显着的味觉反应。味觉神经元的平均自发速率为4.1 ± 5.8（SD）峰/秒。平均响应幅度如下：蔗糖，10.6 +/- 11.7; NaCl，8.6 +/- 14.6;柠檬酸，6.2 +/- 7.8;和QHCl，2.4 +/- 6.6峰值/秒。2.根据它们的最大反应，42个味觉神经元被分类为蔗糖最好，25个NaCl最好，30个柠檬酸最好，4个QHCl最好。这些类别的平均自发速率为蔗糖最佳细胞4.9 +/- 6.2，NaCl最佳细胞5.8 +/- 7.4，柠檬酸最佳细胞1.6 +/- 2.0，QHCl最佳细胞5.8 +/- 6.0峰/秒。柠檬酸最佳神经元的自发放电率显著低于其他类别的神经元。3.在标准浓度下，45个味细胞（44.6%）只对一种味觉刺激有显着反应。在30个酸最好的神经元中，23个（76.7%）只对柠檬酸有反应。对于蔗糖最好的细胞，特异性敏感性较不常见（18/42，42.9%），而对于NaCl最好的神经元，这是相对罕见的（3/25，12%）。4个QHCl最佳神经元中有一个是特异性的。在浓度系列中，超过一半的19个特定的神经元在任何强度下都只对一种化学物质产生反应。4.所有味觉神经元兴奋反应的平均熵为0.60。柠檬酸最好的细胞表现出最小的反应宽度（0.49），蔗糖最好的细胞有点广泛（0.56），但NaCl最好的和QHCl最好的细胞是相当少的选择性（0.77和0.79，分别）。很少观察到抑制，从未达到显著性标准。5.在分层聚类分析中，四个最大的聚类主要通过最佳刺激类别来分离神经元。这是一个主要的例外是一组蔗糖最好的神经元，也响应NaCl和NaCl最好的神经元分组。在二维空间中，特定的味觉神经元，那些只对四种标准味觉刺激中的一种做出反应的神经元，仍然处于分离良好的组中。然而，这些特定的神经元群被其他神经元连接成环状，这些神经元对不止一种的刺激做出反应。（400字处截断摘要）
1. The activity of 117 single neurons was recorded in the rostral nucleus of the solitary tract (NST) and tested with each of four standard chemical stimuli [sucrose, NaCl, citric acid, and quinine HCl (QHCl)] and distilled water in awake, behaving rats. In 101 of these neurons, at least one sapid stimulus elicited a significant taste response. The mean spontaneous rate of the taste neurons was 4.1 +/- 5.8 (SD) spike/s. The mean response magnitudes were as follows: sucrose, 10.6 +/- 11.7; NaCl, 8.6 +/- 14.6; citric acid, 6.2 +/- 7.8; and QHCl, 2.4 +/- 6.6 spikes/s. 2. On the basis of their largest response, 42 taste neurons were classified as sucrose-best, 25 as NaCl-best, 30 as citric acid-best, and 4 as QHCl-best. The mean spontaneous rates for these categories were 4.9 +/- 6.2 for sucrose-best cells, 5.8 +/- 7.4 for NaCl-best, 1.6 +/- 2.0 for citric acid-best, and 5.8 +/- 6.0 spikes/s for QHCl-best. The spontaneous rate of the citric acid-best neurons was significantly lower than that of the other categories. 3. At the standard concentrations, 45 taste cells (44.6%) responded significantly to only one of the gustatory stimuli. Of the 30 acid-best neurons, 23 (76.7%) responded only to citric acid. For sucrose-best cells, specific sensitivity was less common (18/42, 42.9%), and for NaCl-best neurons, it was relatively uncommon (3/25, 12%). One of the 4 QHCl-best neurons was specific. In a concentration series, more than one-half of the 19 specific neurons tested responded to only one chemical at any strength. 4. The mean entropy for the excitatory responses of all gustatory neurons was 0.60. Citric acid-best cells showed the least breadth of responsiveness (0.49), sucrose-best cells were somewhat broader (0.56), but NaCl-best and QHCl-best cells were considerably less selective (0.77 and 0.79, respectively). Inhibition was observed infrequently and never reached the criterion for significance. 5. In the hierarchical cluster analysis, the four largest clusters segregated neurons primarily by best-stimulus category. The major exception to this was a group of sucrose-best neurons that also responded to NaCl and were grouped with the NaCl-best neurons. In a two-dimensional space, the specific taste neurons, those that responded to only one of the four standard sapid stimuli, remained in well-separated groups. These specific groups, however, were joined in a ring-like formation by other neurons that responded to more than one of the sapid stimuli.(ABSTRACT TRUNCATED AT 400 WORDS)