Wnt/β-catenin and LIF-Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal.
Wnt/β-catenin and LIF-Stat3 signaling pathways converge on Sp5 to promote mouse embryonic stem cell self-renewal.
复制标题
Wnt/β-catenin和LIF-Stat3信号通路汇聚在Sp5上促进小鼠胚胎干细胞自我更新
DOI：
10.1242/jcs.177675
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发表时间：
2016-01-15
影响因子：
4
通讯作者：
Ying QL
中科院分区：
文献类型：
--
作者：
Ye S;Zhang D;Cheng F;Wilson D;Mackay J;He K;Ban Q;Lv F;Huang S;Liu D;Ying QL
激活白血病抑制因子-STAT3或Wnt/β-catenin信号通路可促进小鼠胚胎干细胞的自我更新。在单独的信号通路中已经发现了无数的下游靶点，但它们的共同靶点在很大程度上仍然难以捉摸。在本研究中，我们发现LIF-STAT3和Wnt/β-catenin信号通路在Sp5上汇聚，促进mESC的自我更新。强制表达sp5可以复制Wnt/β-catenin信号的部分作用，但模仿LIF-STAT3信号的大部分特征以维持未分化的mESCs。此外，SP5能够将小鼠上皮细胞干细胞转化为幼稚的多能状态。因此，SP5是调控mESC幼稚多能性的调控网络的重要组成部分。摘要：本研究揭示了Sp5在CHIR99021和LIF介导的小鼠胚胎干细胞自我更新中的新功能，以及将EpiSCs重新编程为NAїve ESCs。
Activation of leukemia inhibitor factor (LIF)–Stat3 or Wnt/β-catenin signaling promotes mouse embryonic stem cell (mESC) self-renewal. A myriad of downstream targets have been identified in the individual signal pathways, but their common targets remain largely elusive. In this study, we found that the LIF–Stat3 and Wnt/β-catenin signaling pathways converge on Sp5 to promote mESC self-renewal. Forced Sp5 expression can reproduce partial effects of Wnt/β-catenin signaling but mimics most features of LIF–Stat3 signaling to maintain undifferentiated mESCs. Moreover, Sp5 is able to convert mouse epiblast stem cells into a naïve pluripotent state. Thus, Sp5 is an important component of the regulatory network governing mESC naïve pluripotency. Summary: This study reveals a new function of Sp5 in mouse embryonic stem cell (ESC) self-renewal mediated by CHIR99021 and LIF, and reprogramming of EpiSCs into naїve ESCs.