Development of proximal coronary arteries in quail embryonic heart - Multiple capillaries penetrating the aortic sinus fuse to form main coronary trunk
Development of proximal coronary arteries in quail embryonic heart - Multiple capillaries penetrating the aortic sinus fuse to form main coronary trunk
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DOI：
10.1161/01.res.0000112963.79064.09
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发表时间：
2004-02-20
影响因子：
20.1
通讯作者：
Nakamura, H
中科院分区：
文献类型：
--
作者：
Ando, K;Nakajima, Y;Nakamura, H
研究表明，近端冠状动脉（PCA）是通过冠状动脉血管系统的环周（PR）内皮向内生长而形成的。然而，五氯苯甲醚形成的细节仍不清楚。我们使用QH 1（鹌鹑内皮标记物）和平滑肌α-肌动蛋白的双重免疫染色，检查了孵育5至9天（胚胎日[艾德]）的鹌鹑胚胎心脏中PCA的发育。在6至7艾德，几个QH 1阳性内皮链从PR穿透对面的窦，在一些胚胎中，几个内皮链穿透后（非冠状）窦。在7 - 8艾德时，穿透对面窦的内皮链似乎融合，形成近端冠状动脉干，通过冠状动脉的新生平滑肌层与主动脉壁分界。到9艾德时，两个冠状动脉干完全形成，先前穿透非冠状窦的内皮束消失。在6艾德的共聚焦显微镜显示不连续的QH 1阳性内皮祖细胞在主动脉壁的网站，内皮股后来发展。观察结果表明，在PCA的形成过程中，来自PR的内皮股穿透面对的窦，然后融合，而穿透非冠状窦的那些股消失。此后，冠状动脉图尼卡中膜将确定的PCA与主动脉中膜区分开来。
Studies have shown that the proximal coronary artery (PCA) develops via endothelial ingrowth from the peritruncal ring (PR) of the coronary vasculature. However, the details of PCA formation remain unclear. We examined the development of PCAs in quail embryonic hearts from 5 to 9 days of incubation (embryonic day [ED]) using double-immunostaining for QH1 (quail endothelial marker) and smooth muscle alpha-actin. At 6 to 7 ED, several QH1-positive endothelial strands from the PR penetrated the facing sinuses, and in some embryos, several endothelial strands penetrated the posterior (noncoronary) sinus. At 7 to 8 ED, the endothelial strands penetrating the facing sinuses seemed to fuse, forming a proximal coronary stem that was demarcated from the aortic wall by the nascent smooth muscle layer of the coronary artery. By 9 ED, two coronary stems were completely formed, and the endothelial strands previously penetrating the noncoronary sinus had disappeared. Confocal microscopy at 6 ED revealed discontinuous QH1-positive endothelial progenitors in the aortic wall at sites where the endothelial strands would later develop. Observations demonstrate that during the formation of the PCA, endothelial strands from the PR penetrate the facing sinuses and then fuse, whereas those strands penetrating the noncoronary sinus disappear. Thereafter, the coronary artery tunica media demarcates the definitive PCA from the aortic media.