STUDIES ON HISTOCHEMISTRY AND EXTRACTION OF CRASSULA OVATA (MILL.) DRUCE POLYSACCHARIDES
STUDIES ON HISTOCHEMISTRY AND EXTRACTION OF CRASSULA OVATA (MILL.) DRUCE POLYSACCHARIDES
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景天果多糖的组织化学及提取研究
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影响因子：
0.3
通讯作者：
SHUPENG WU
中科院分区：
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作者：
赵猛;JING KANG;YATING ZHAO;SHUPENG WU
卵叶厚壳Crassula ovata（Mill.）Druce属于景天科。本文研究了卵叶厚壳Crassula ovata（Mill.）德鲁斯被研究过。采用组织化学方法结合PAS反应确定多糖的贮存部位。结果表明：卵叶厚壳Crassula ovata（Mill.）叶由表皮细胞和叶肉细胞组成。茎的结构包括表皮、皮层和维管。PAS反应表明，多糖主要贮存在茎皮层细胞和叶片叶肉组织中。采用正交试验法对卵叶厚壳多糖的提取工艺进行优化。德鲁斯走了最佳提取工艺条件为：料液比1：10，提取时间120 min，提取温度60 ℃，乙醇浓度70%，醇沉30 min。
Crassula ovata (Mill.) Druce belongs to the family of Crassulaceae. In the present study, The microstructure of the leaves and stem of Crassula ovata (Mill.) Druce was studied. Samples were processed for light microscopy, and the storage site of polysaccharide was determined by histochemical method with PAS reaction. The results showed that Crassula ovata (Mill.) Druce leaves were composed of epidermal and mesophyll cells. The structure of stems were included epidermis, cortex and vascular. PAS reaction showed that polysaccharides mainly store in the stem cortex cells and leaf mesophyll tissue. Orthogonal design was used to optimize experimental conditions for crude polysaccharides from Crassula ovata (Mill.) Druce leaves. The optimum extraction conditions were as follows: ratio of material to liquid 1:10, extraction time 120 min, extraction temperature 60 ℃, ethanol concentration 70%, ethanol precipitation for 30 min.