CORRELATION OF DNA ADDUCT LEVELS IN HUMAN-LUNG WITH CIGARETTE-SMOKING
CORRELATION OF DNA ADDUCT LEVELS IN HUMAN-LUNG WITH CIGARETTE-SMOKING
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DOI：
10.1038/336790a0
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发表时间：
1988-12-22
期刊：
影响因子：
64.8
通讯作者：
KING, MM
中科院分区：
文献类型：
--
作者：
PHILLIPS, DH;HEWER, A;KING, MM
肺癌是英国男性最常见的癌症，女性第二常见的癌症，占所有癌症死亡的25%至40% 1，2吸烟被广泛认为是肺癌的主要原因，吸烟数量与肺癌风险之间存在线性关系3。虽然在香烟烟雾中已经发现了大约50种致癌化学物质4，但尚未确定特定化合物与肺癌之间的因果关系。对吸烟者的尿液5、6、血液7 - 11和胎盘12的研究提供了致癌物暴露的迹象，尽管人类DNA中存在共价结合加合物提供了致癌物暴露的证据12 -14，但尚未有关于人类肺中DNA加合物水平的系统研究报告。我们在这里报告，使用32 P-后标记技术15，16，吸烟者比非吸烟者有更高的加合物水平，加合物水平与每日或终身吸烟量之间存在线性关系，戒烟至少五年的人与非吸烟者的加合物水平相似。
Lung cancer is the most common cancer in men in the United Kingdom and the second most common in women, accounting for between 25 and 40% of all cancer deaths1,2Cigarette smoking is widely accepted as the major cause of lung cancer and linear relationships have been established between the number of cigarettes smoked and lung cancer risk3. Although approximately 50 carcinogenic chemicals have been identified in cigarette smoke4, a causal link between specific compounds and lung cancer has yet to be made. Studies on cigarette smokers' urine5,6, blood7–11and placenta12have provided indications of carcinogen exposure, and although the presence of covalently-bound adducts in human DNA provides evidence of exposure to carcinogens12–14, there have been no reports of systematic studies on the levels of DNA adducts in human lung. We report here, using the32P-post-labelling technique15,16, that cigarette smokers have higher adduct levels than non-smokers, that there is a linear relationship between adduct levels and daily or lifetime cigarette consumption, and that people who have given up smoking for at least five years have adduct levels similar to those of non-smokers.