Somatotopic Organization and Temporal Characteristics of Cerebrocortical Excitation in Response to Nasal Mucosa Stimulation With and Without an Odor in the Rat: An Optical Imaging Study
Somatotopic Organization and Temporal Characteristics of Cerebrocortical Excitation in Response to Nasal Mucosa Stimulation With and Without an Odor in the Rat: An Optical Imaging Study
复制标题
大鼠有或没有气味时鼻粘膜刺激反应的脑皮质兴奋的体位组织和时间特征：光学成像研究
DOI：
10.1016/j.neuroscience.2018.02.042
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发表时间：
2018
期刊：
影响因子：
3.3
通讯作者：
Kobayashi Masayuki
中科院分区：
文献类型：
--
作者：
Zama Manabu;Hara Yaeko;Fujita Satoshi;Kaneko Tadayoshi;Kobayashi Masayuki
鼻黏膜在加热和加湿吸入空气中起作用，对机械刺激高度敏感。此外，鼻黏膜上部表达处理嗅觉信息的嗅觉受体。虽然面部初级（S1）和次级（S2）体感皮层的体感图谱已被清楚地记录下来，但鼻黏膜的体感图谱以及气味对其活动的影响在很大程度上是未知的。本研究旨在利用光学成像技术鉴定大鼠鼻腔粘膜体感刺激下S1皮质区及其时间特征。鼻腔粘膜的充气应用反应首先发生在对侧S1的一部分，随后向喙侧和腹侧邻近部位扩散。上咽刺激最初激活了这一喙侧扩张部位，从最初激活的区域向腹侧区域的兴奋传播可能代表S2。信号强度和激活面积随气压增大而增大。鼻尖刺激最初刺激的S1区位于鼻粘膜的尾侧。此外，在有和没有乙酸戊酯气味的鼓气刺激中，S1兴奋的振幅相似。与对侧S1相比，气味吹气刺激在同侧梨状皮质显示微弱的光信号增加。这些结果表明，来自鼻黏膜、皮肤和上咽的体感信息在S1的空间连续区域被处理，而对侧S1的体感和嗅觉系统之间的相互作用相对较小。
Nasal mucosa has roles in warming and humidifying inspired air and is highly sensitive to mechanical stimuli. Moreover, the upper part of the nasal mucosa expresses olfactory receptors processing olfactory information. Although the somatosensory map of the face in the primary (S1) and secondary (S2) somatosensory cortices is clearly documented, the map of the nasal mucosa and the effect of odors on their activities are largely unknown. This study aimed to identify the cortical regions in S1 and their temporal features in response to somatosensory stimulation of the nasal mucosa using an optical imaging technique in urethane-anesthetized rats. An air puff application response to nasal mucosa first occurred in a part of contralateral S1 and subsequently, spread toward the rostrally and ventrally adjacent sites. Upper pharynx stimulation initially activated this rostrally expanded site and the excitatory propagation from the initially activated region toward ventral region likely represented S2. Signal intensity and activated area increased dependent on air pressure. Nasal tip stimulation initially excited S1 region caudally adjacent to that of nasal mucosa. Moreover, the amplitude of S1 excitation was similar between air puff stimulation with and without an odor, amyl acetate. In contrast to contralateral S1, air puff stimulation with the odor showed a faint optical signal increase in the ipsilateral piriform cortex. These results suggest that somatosensory information from the nasal mucosa and skin, and upper pharynx are processed in spatially continuous regions of S1, and interaction between somatosensory and olfactory systems is relatively small in contralateral S1.