Comparison of the characteristics of four metallochromic dyes as potential calcium indicators for biological experiments.
Comparison of the characteristics of four metallochromic dyes as potential calcium indicators for biological experiments.
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四种金属变色染料作为生物实验潜在钙指示剂的特性比较。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
N. Kurebayashi
中科院分区：
文献类型：
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作者：
Y. Ogawa;H. Harafuji;N. Kurebayashi
1.研究了4种钙指示剂（紫脲酸盐（MX）、四甲基紫脲酸盐（TMX）、偶氮胂Ⅲ（Az）和偶氮安替比林Ⅲ（Ap））的特性，以评价它们在生物实验中的适用性。2. Az虽然对Ca有很高的灵敏度，但也有以下严重的缺点：（i）由于其络合物结构组成的变化，吸光度随Ca浓度的变化呈非线性（2：1复合物至1：1复合物），并且由于在20 μ M或更高的Ca 2+下与Ca的高表观结合常数;（ii）严重的干扰Mg 2 +;（iii）相当低的协会和解离速率常数与钙是不够的条件下，其中Ca 2+被确定在系统中不能被Az干扰;（iv）抑制作用牛蛙破碎的肌浆网对钙的吸收。3. Ap具有类似的缺点，尽管Ap比Az更快地与Ca反应。(i)Ap受到Mg 2+的干扰，即使Mg 2+的影响可以以降低灵敏度为代价而最小化。(ii)吸光度变化对Ca浓度的曲线在20 μ M Ca 2+或更高时偏离线性。(iii)Ap在不干扰待测体系中Ca 2+的浓度下不是很灵敏。TMX的灵敏度是MX的两倍。然而，在存在ATP的情况下，TMX可能比MX更容易透过膜。MX除了对Ca ~（2+）的敏感性低外，没有其他缺点。
1. The characteristics of four calcium indicators, murexide(MX), tetramethylmurexide(TMX), arsenazo III (Az) and antipyrylazo III (Ap), were examined to assess their applicability for biological experiments. 2. Az has the following serious disadvantages, although it has a high sensitivity to Ca: (i) nonlinearity of absorbance change against the concentration of Ca due to a change in the structural composition of its complex (2 : 1 complex to 1 : 1 complex) in the range below 10 micro M Ca2+, and due to the high apparent binding constant to Ca at 20 micro M or higher Ca2+; (ii) severe interference by Mg2+; (iii) rather low association and dissociation rate constants with Ca which are not sufficient under conditions where Ca2+ to be determined in the system cannot be disturbed by Az; (iv) inhibitory effect on Ca uptake by bullfrog fragmented sarcoplasmic reticulum. 3. Ap has similar disadvantages, although Ap reacts with Ca faster than Az. (i) Ap suffers from interference by Mg2+ even though the effect of Mg2+ can be minimized at the expense of reduced sensitivity. (ii) Plots of absorbance change versus the concentration of Ca deviate from linearity around 20 micro M Ca2+ or higher. (iii) Ap is not very sensitive at concentrations which would not disturb Ca2+ in the system to be determined. TMX is twice as sensitive as MX. However, TMX may be more permeable through membranes than MX in the presence of ATP. MX has no disadvantages except for its low sensitivity to Ca2+.