Biotransformation of arsenic-containing roxarsone by an aerobic soil bacterium Enterobacter sp. CZ-1
Biotransformation of arsenic-containing roxarsone by an aerobic soil bacterium Enterobacter sp. CZ-1
复制标题
需氧土壤细菌肠杆菌对含砷洛克沙胂的生物转化。
DOI：
10.1016/j.envpol.2019.01.076
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发表时间：
2019-04-01
影响因子：
8.9
通讯作者：
Zhao, Fang-Jie
中科院分区：
文献类型：
--
作者：
Huang, Ke;Peng, Hanyong;Zhao, Fang-Jie
Roxarson（3-硝基-4-羟基苯胂酸，ROX）是一种含砷化合物，广泛用作家禽业的饲料添加剂。鸡粪中排出的ROX可以被微生物转化为环境中不同的砷形态。迄今为止，大多数关于ROX微生物转化的研究都集中在厌氧微生物上。在这里，我们从砷污染的水稻土中分离出纯培养的好氧 ROX 转化菌株 CZ-1。根据16S rRNA基因序列，菌株CZ-1被分类为肠杆菌属的成员。菌株CZ-1在ROX生物转化过程中，基于高性能液体检测并鉴定了砷酸盐（As[V]）、亚砷酸盐（As[III]）、N-乙酰基-4-羟基-间胂酸（N-AHPAA）、3-氨基-4-羟基苯胂酸（3-AHPAA）和一种新型含硫砷物质（AsC9H13N2O6S）等5种代谢物。色谱-电感耦合等离子体质谱 (HPLC-ICP-MS)、HPLC-ICP-MS/电喷雾电离质谱 (ESI-MS) 和 HPLC-电喷雾电离混合四极杆飞行时间质谱 (ESI-qTOE-MS) 分析。主要产品为N-AHPAA和3-AHPAA，3-AHPAA也可转化为N-AHPAA。基于这些结果，我们提出了一种新的肠杆菌 ROX 生物转化途径。 sp CZ-1，其中ROX的硝基首先被还原为氨基(3-AHPAA)，然后乙酰化为N-AHPAA。 (C) 2019 Elsevier Ltd. 保留所有权利。
Roxarsone (3-nitro-4-hydroxyphenylarsonic acid, ROX) is an arsenic-containing compound widely used as a feed additive in poultry industries. ROX excreted in chicken manure can be transformed by microbes to different arsenic species in the environment. To date, most of the studies on microbial transformation of ROX have focused on anaerobic microorganisms. Here, we isolated a pure cultured aerobic ROX-transforming bacterial strain, CZ-1, from an arsenic-contaminated paddy soil. On the basis of 16S rRNA gene sequence, strain CZ-1 was classified as a member of the genus Enterobacter. During ROX biotransformation by strain CZ-1, five metabolites including arsenate (As[V]), arsenite (As[III]), N-acetyl-4-hydroxy-m-arsanilic acid (N-AHPAA), 3-amino-4-hydroxyphenylarsonic acid (3-AHPAA) and a novel sulfur-containing arsenic species (AsC9H13N2O6S) were detected and identified based on high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS), HPLC-ICP-MS/electrospray ionization mass spectrometry (ESI-MS) and HPLC-electrospray ionization hybrid quadrupole time-of-flight mass spectrometry (ESI-qTOE-MS) analyses. N-AHPAA and 3-AHPAA were the main products, and 3-AHPAA could also be transformed to N-AHPAA. Based on the results, we propose a novel ROX biotransformation pathway by Enterobacter. sp CZ-1, in which the nitro group of ROX is first reduced to amino group (3-AHPAA) and then acetylated to N-AHPAA. (C) 2019 Elsevier Ltd. All rights reserved.