Isolation of a novel strain of Butyrivibrio fibrisolvens that isomerizes linoleic acid to conjugated linoleic acid without hydrogenation, and its utilization as a probiotic for animals
Isolation of a novel strain of Butyrivibrio fibrisolvens that isomerizes linoleic acid to conjugated linoleic acid without hydrogenation, and its utilization as a probiotic for animals
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DOI：
10.1111/j.1365-2672.2006.02864.x
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发表时间：
2006-04-01
影响因子：
4
通讯作者：
Hino, T
中科院分区：
文献类型：
--
作者：
Fukuda, S;Suzuki, Y;Hino, T
目的：分离出一株具有强大共轭亚油酸（CLA）生产能力的新的溶纤维丁酸弧菌菌株，以便用作动物益生菌。
方法与结果：从山羊瘤胃中分离出一株新菌株（MDT - 5），由于其具有高亚油酸异构酶活性且几乎无CLA还原酶活性，它能将亚油酸（LA）专一性地转化为CLA。MDT - 5还能将亚麻酸转化为共轭亚麻酸，共轭亚麻酸可能比CLA具有更强的生物活性。每隔一天给小鼠口服MDT - 5，持续2周，导致大肠内容物中CLA含量增加（2.5倍），脂肪组织中CLA含量也增加（3倍）。喂食高亚油酸饮食以及延长MDT - 5给药时间，进一步增加了体脂中的CLA含量。
结论：MDT - 5生产CLA的能力远远强于任何其他已知细菌。给小鼠施用MDT - 5可增加大肠中CLA的产生，从而导致CLA吸收增加。
研究的意义与影响：MDT - 5作为一种益生菌可用于宠物动物，持续提供CLA。
Aim: Isolation of a new strain of Butyrivibrio fibrisolvens possessing great capacity to produce conjugated linoleic acid (CLA) in order to utilize as a probiotic for animals.Methods and Results: A novel strain (MDT-5) was isolated from the goat rumen, which exclusively converted linoleic acid (LA) to CLA, because of its high LA isomerase activity with virtually no CLA reductase activity. MDT-5 also converted linolenic acid to conjugated linolenic acid that may be more bioactive than CLA. The oral administration of MDT-5 every other day to mice for 2 weeks resulted in increased amounts of CLA in the contents of the large intestine (2.5-fold), as well as in adipose tissue (threefold). Feeding a high-LA diet, as well as prolonging the period of MDT-5 administration, further increased the CLA content in body fat.Conclusions: MDT-5 has by far greater ability to produce CLA than any other known bacteria. Administration of MDT-5 to mice increases CLA production in the large intestine, which results in increased CLA absorption.Significance and Impact of the Study: MDT-5 may be useful in pet animals as a probiotic to provide CLA continuously.