Purification of the Vibrio Quorum-Sensing Transcription Factors LuxR, HapR, and SmcR.
Purification of the Vibrio Quorum-Sensing Transcription Factors LuxR, HapR, and SmcR.
复制标题
弧菌群体感应转录因子 LuxR、HapR 和 SmcR 的纯化。
DOI：
10.1007/7651_2020_306
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
vanKessel,JuliaC
中科院分区：
文献类型：
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作者：
Newman,JaneD;vanKessel,JuliaC
群体感应是细菌之间细胞通信的一种依赖于细胞密度的形式。由于弧菌具有多样且复杂的表型以及与人类和水产养殖疾病的相关性，这种信号传导过程已在弧菌中得到了深入研究。弧菌群体感应的机制研究需要优化蛋白质纯化技术来检查关键蛋白质的作用，例如控制群体感应基因表达的转录因子 LuxR/HapR 家族。蛋白质纯化是生物化学的基石，持续生产批量的纯净、活性和浓缩的蛋白质以进行各种分析至关重要。此处描述的方法针对 Vibriomaster 群体感应调节器 LuxR（哈维弧菌）、HapR（霍乱弧菌）和 SmcR（创伤弧菌）的纯化进行了优化。我们预计这些方法可以应用于该转录因子家族中的其他蛋白质。
Quorum sensing is a cell density-dependent form of cellular communication among bacteria. This signaling process has been heavily studied in vibrios due to their diverse and complex phenotypes and relevance to human and aquaculture disease. Mechanistic studies ofVibrioquorum sensing have required optimization of protein purification techniques to examine the role of key proteins, such as the LuxR/HapR family of transcription factors that control quorum-sensing gene expression. Protein purification is the cornerstone of biochemistry, and it is crucial to consistently produce batches of protein that are pure, active, and concentrated to perform various assays. The methods described here are optimized for purification of theVibriomaster quorum-sensing regulators, LuxR (Vibrio harveyi), HapR (Vibrio cholerae), and SmcR (Vibrio vulnificus). We anticipate that these methods can be applied to other proteins in this family of transcription factors.